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Comparative Genomics Screens Identify a Novel Small Secretory Peptide, SlSolP12, which Activates Both Local and Systemic Immune Response in Tomatoes and Exhibits Broad-Spectrum Activity.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2024 Aug 21; Vol. 72 (33), pp. 18507-18519. Date of Electronic Publication: 2024 Aug 07. - Publication Year :
- 2024
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Abstract
- Small secreted peptides (SSPs) are essential for defense mechanisms in plant-microbe interactions, acting as danger-associated molecular patterns (DAMPs). Despite the first discovery of SSPs over three decades ago, only a limited number of SSP families, particularly within Solanaceae plants, have been identified due to inefficient approaches. This study employed comparative genomics screens with Solanaceae proteomes (tomato, tobacco, and pepper) to discover a novel SSP family, SolP. Bioinformatics analysis suggests that SolP may serve as an endogenous signal initiating the plant PTI response. Interestingly, SolP family members from tomato, tobacco, and pepper share an identical sequence (VTSNALALVNRFAD), named SlSolP12 (also referred to as NtSolP15 or CaSolP1). Biochemical and phenotypic analyses revealed that synthetic SlSolP12 peptide triggers multiple defense responses: ROS burst, MAPK activation, callose deposition, stomatal closure, and expression of immune defense genes. Furthermore, SlSolP12 enhances systemic resistance against Botrytis cinerea infection in tomato plants and interferes with classical peptides, flg22 and Systemin, which modulate the immune response. Remarkably, SolP12 activates ROS in diverse plant species, such as Arabidopsis thaliana , soybean, and rice, showing a broad spectrum of biological activities. This study provides valuable approaches for identifying endogenous SSPs and highlights SlSolP12 as a novel DAMP that could serve as a useful target for crop protection.
- Subjects :
- Peptides immunology
Peptides chemistry
Disease Resistance genetics
Disease Resistance immunology
Gene Expression Regulation, Plant
Nicotiana immunology
Nicotiana genetics
Nicotiana microbiology
Nicotiana metabolism
Capsicum immunology
Capsicum genetics
Capsicum microbiology
Capsicum chemistry
Solanum lycopersicum immunology
Solanum lycopersicum genetics
Solanum lycopersicum microbiology
Plant Proteins genetics
Plant Proteins immunology
Plant Proteins metabolism
Genomics
Plant Diseases microbiology
Plant Diseases immunology
Plant Diseases genetics
Plant Immunity genetics
Botrytis
Subjects
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 72
- Issue :
- 33
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 39113497
- Full Text :
- https://doi.org/10.1021/acs.jafc.4c03633