Back to Search
Start Over
Real-Time RT-PCR Assays for Typing of Epizootic Hemorrhagic Disease Virus.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2838, pp. 163-170. - Publication Year :
- 2024
-
Abstract
- Real-time RT-PCR for the detection of epizootic hemorrhagic disease virus (EHDV) in clinical samples is a fast and sensitive tool for the diagnosis and confirmation of disease. Several real-time RT-PCR methods have been reported over the last 10 years. In this chapter, we describe seven duplex real-time RT-PCR assays to amplify part of genome segment 2 of EHDV to enable serotype identification. The assay includes the detection of an endogenous control gene-beta-actin.<br /> (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
- Subjects :
- Animals
Reoviridae Infections veterinary
Reoviridae Infections virology
Reoviridae Infections diagnosis
RNA, Viral genetics
Hemorrhagic Disease Virus, Epizootic genetics
Hemorrhagic Disease Virus, Epizootic isolation & purification
Hemorrhagic Disease Virus, Epizootic classification
Real-Time Polymerase Chain Reaction methods
Reverse Transcriptase Polymerase Chain Reaction methods
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2838
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 39126631
- Full Text :
- https://doi.org/10.1007/978-1-0716-4035-7_11