Back to Search
Start Over
Overexpression of Extracellular Superoxide Dismutase 3 Inhibits Cancer Cell Growth and Migration in Colorectal Cancer.
- Source :
-
The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology [Turk J Gastroenterol] 2024 Jun; Vol. 35 (6), pp. 465-474. - Publication Year :
- 2024
-
Abstract
- Background/aims:  Incidence of colorectal cancer is rapidly increasing worldwide. Extracellular superoxide dismutase (EcSOD; SOD3) is an antioxidant enzyme. However, SOD3 roles in colorectal cancer progression remain uncertain.<br />Materials and Methods:  Superoxide dismutase 3 expression was evaluated, and we analyzed clinical relevance of SOD3 expression in colorectal cancer. Subsequently, SOD3 roles in colorectal cancer progression were detected by gain of function experiments. Changes in subcutaneous tumor and liver nodule size after SOD3 overexpression were examined in nude mice. The expression of proliferation marker Ki67 was assessed by immunohistochemical staining.<br />Results:  Supperoxide dismutase 3 was downregulated in colorectal cancer (P <.01). Downregulation of SOD3 was correlated with unfavorable outcomes (P < .05). Superoxide dismutase 3 upregulation limited the proliferative (P <.05), migrative (P <.01) and invasive actions of colorectal cancer cells (P <.01) by suppressing epithelial-mesenchymal transition. Moreover, SOD3 overexpression reduced Ki67 expression (P <.01) and blocked tumor growth (P <01) and liver metastasis (P <.001) in mouse tumor model.<br />Conclusion:  Superoxide dismutase 3 upregulation attenuates tumor growth and liver metastasis in colorectal cancer, suggesting that SOD3 has potential diagnostic and prognostic values regarding colorectal cancer treatment.
- Subjects :
- Animals
Female
Humans
Male
Mice
Middle Aged
Cell Line, Tumor
Disease Progression
Down-Regulation
Ki-67 Antigen metabolism
Liver Neoplasms pathology
Mice, Inbred BALB C
Mice, Nude
Up-Regulation
Cell Movement
Cell Proliferation
Colorectal Neoplasms pathology
Colorectal Neoplasms genetics
Epithelial-Mesenchymal Transition
Superoxide Dismutase metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2148-5607
- Volume :
- 35
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 39128081
- Full Text :
- https://doi.org/10.5152/tjg.2024.23232