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Gossypium arboreum PPD2 facilitates root architecture development to increase plant resilience to salt stress.
- Source :
-
Physiologia plantarum [Physiol Plant] 2024 Jul-Aug; Vol. 176 (4), pp. e14473. - Publication Year :
- 2024
-
Abstract
- The jasmonic acid (JA) signaling pathway plays an important role in plant responses to abiotic stresses. The PEAPOD (PPD) and jasmonate ZIM-domain (JAZ) protein in the JA signaling pathway belong to the same family, but their functions in regulating plant defense against salt stress remain to be elucidated. Here, Gossypium arboreum PPD2 was overexpressed in Arabidopsis thaliana and systematically silenced in cotton for exploring its function in regulating plant defense to salt stress. The GaPPD2-overexpressed Arabidopsis thaliana plants significantly increased the tolerance to salt stress compared to the wild type in both medium and soil, while the GaPPD2-silenced cotton plants showed higher sensitivity to salt stress than the control in pots. The antioxidant activities experiment showed that GaPPD2 may mitigate the accumulation of reactive oxygen species by promoting superoxide dismutase accumulation, consequently improving plant resilience to salt stress. Through the exogenous application of MeJA (methy jasmonate) and the protein degradation inhibitor MG132, it was found that GaPPD2 functions in plant defense against salt stress and is involved in the JA signaling pathway. The RNA-seq analysis of GaPPD2-overexpressed A. thaliana plants and receptor materials showed that the differentially expressed genes were mainly enriched in antioxidant activity, peroxidase activity, and plant hormone signaling pathways. qRT-PCR results demonstrated that GaPPD2 might positively regulate plant defense by inhibiting GH3.2/3.10/3.12 expression and activating JAZ7/8 expression. The findings highlight the potential of GaPPD2 as a JA signaling component gene for improving the cotton plant resistance to salt stress and provide insights into the mechanisms underlying plant responses to environmental stresses.<br /> (© 2024 Scandinavian Plant Physiology Society.)
- Subjects :
- Plants, Genetically Modified
Salt Tolerance genetics
Plant Growth Regulators metabolism
Signal Transduction drug effects
Gossypium genetics
Gossypium physiology
Gossypium drug effects
Cyclopentanes metabolism
Cyclopentanes pharmacology
Oxylipins metabolism
Oxylipins pharmacology
Plant Proteins genetics
Plant Proteins metabolism
Arabidopsis genetics
Arabidopsis physiology
Arabidopsis drug effects
Plant Roots genetics
Plant Roots growth & development
Plant Roots physiology
Plant Roots drug effects
Gene Expression Regulation, Plant drug effects
Salt Stress
Subjects
Details
- Language :
- English
- ISSN :
- 1399-3054
- Volume :
- 176
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Physiologia plantarum
- Publication Type :
- Academic Journal
- Accession number :
- 39129661
- Full Text :
- https://doi.org/10.1111/ppl.14473