Comparative transcriptomic analysis of hepatopancreas reveals that more genes are involved in the exposure response of Vibrio parahaemolyticus PirA vp compared to PirB vp .

Vibrio parahaemolyticus (VP- _AHPND ) is regarded as one of the main pathogens that caused acute hepatopancreatic necrosis disease (AHPND) in the Pacific white shrimp Litopenaeus vannamei. PirA ^vp and PirB ^vp toxin proteins are the main pathogenic proteins of AHPND in shrimp. Knowledge about the mechanism of shrimp response to PirA ^vp or PirB ^vp toxin is very helpful for developing new prevention and control strategy of AHPND in shrimp. In this study, the pathological sections showed that after 4 h treatment, significant pathological changes were observed in the PirB ^vp treated group, and no obvious pathological changes was found in PirA ^vp treated group. In order to learn the mechanism of shrimp response to PirA ^vp and PirB ^vp , comparative transcriptome was applied to analyze the different expressions of genes in the hepatopancreas of shrimp after treatment with PirA ^vp or PirB ^vp . A total of 9978 differentially expressed genes (DEGs) were identified between PirA ^vp or PirB ^vp -treated and PBS control shrimp, including 6616 DEGs in the PirA ^vp treated group and 3362 DEGs in the PirB ^vp treated group. There were 2263 DEGs that were commonly expressed, 4353 DEGs were only expressed in PirA ^vp VS PBS group and 1099 DEGs were uniquely expressed in PirB ^vp VS PBS group. Among these DEGs, the anti-apoptosis related pathways and immune response related genes significantly expressed in the commonly expressed DEGs of PirA ^vp VS PBS group and PirB ^vp VS PBS group, and small GTPase-mediated signaling and DNA metabolic process might relate to the host special reaction towards PirA ^vp and PirB ^vp exposure. The data suggested that the differential expression of these immune and metabolic-related genes in hepatopancreas might contribute to the pathogenicity variations of shrimp to VP- _AHPND . The identified genes in this study will be useful for clarifying the response mechanism of shrimp toward different toxins of VP- _AHPND and will further provide molecular basis for understanding the pathogenic mechanism of VP- _AHPND .
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