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Persistent changes in nociceptor translatomes govern hyperalgesic priming in mouse models.
- Source :
-
BioRxiv : the preprint server for biology [bioRxiv] 2024 Aug 08. Date of Electronic Publication: 2024 Aug 08. - Publication Year :
- 2024
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Abstract
- Hyperalgesic priming is a model system that has been widely used to understand plasticity in painful stimulus-detecting sensory neurons, called nociceptors. A key feature of this model system is that following priming, stimuli that do not normally cause hyperalgesia now readily provoke this state. We hypothesized that hyperalgesic priming occurs due to reorganization of translation of mRNA in nociceptors. To test this hypothesis, we used paclitaxel treatment as the priming stimulus and translating ribosome affinity purification (TRAP) to measure persistent changes in mRNA translation in Nav1.8+ nociceptors. TRAP sequencing revealed 161 genes with persistently altered mRNA translation in the primed state. We identified Gpr88 as upregulated and Metrn as downregulated. We confirmed a functional role for these genes, wherein a GPR88 agonist causes pain only in primed mice and established hyperalgesic priming is reversed by Meteorin. Our work demonstrates that altered nociceptor translatomes are causative in producing hyperalgesic priming.<br />Competing Interests: Conflict of Interest Statement: G Munro and KA Petersen are employees of Hoba Therapeutics. The authors declare no other conflicts of interest.
Details
- Language :
- English
- ISSN :
- 2692-8205
- Database :
- MEDLINE
- Journal :
- BioRxiv : the preprint server for biology
- Publication Type :
- Academic Journal
- Accession number :
- 39149295
- Full Text :
- https://doi.org/10.1101/2024.08.07.606891