Back to Search
Start Over
Functional Characterization of Odorant Receptors for Sex Pheromone (Z)-11-Hexadecenol in Orthaga achatina .
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2024 Aug 28; Vol. 72 (34), pp. 18864-18871. Date of Electronic Publication: 2024 Aug 17. - Publication Year :
- 2024
-
Abstract
- Pheromone receptor (PR)-mediated transduction of sex pheromones to electrophysiological signals is the basis for sex pheromone communication. Orthaga achatina , a serious pest of the camphor tree, uses a mixture of four components (Z11-16:OAc, Z11-16:OH, Z11-16:Ald, and Z3,Z6,Z9,Z12,Z15-23:H) as its sex pheromone. In this study, we identified five PR genes ( OachPR 1-5) by phylogenetic analysis. Further RT-PCR and qPCR experiments showed that PR 1-3 were specifically expressed in male antennae, while PR 4 was significantly female-biased in expression. Functional characterization using the XOE-TEVC assay demonstrated that PR1 and PR3 both responded strongly to Z11-16:OH, while PR1 and PR3 had a weak response to Z3,Z6,Z9,Z12,Z15-23:H and Z11-16:Ald, respectively. Finally, two key amino acid residues (N78 and R331) were confirmed to be essential for binding of PR3 with Z11-16:OH by molecular docking and site-directed mutagenesis. This study helps understand the sex pheromone recognition molecular mechanism of O. achatina .
- Subjects :
- Animals
Male
Female
Molecular Docking Simulation
Fatty Alcohols metabolism
Fatty Alcohols chemistry
Fatty Alcohols pharmacology
Aldehydes
Sex Attractants chemistry
Sex Attractants metabolism
Receptors, Odorant genetics
Receptors, Odorant metabolism
Receptors, Odorant chemistry
Insect Proteins genetics
Insect Proteins metabolism
Insect Proteins chemistry
Phylogeny
Subjects
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 72
- Issue :
- 34
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 39153187
- Full Text :
- https://doi.org/10.1021/acs.jafc.4c05108