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Bioemulsifier from sponge-associated bacteria reduces staphylococcal biofilm.

Authors :
Dias GR
Freitas-Silva J
de Carvalho MM
Ramos VFDS
Muricy G
Rodrigues JCF
Costa BRFV
de Oliveira BFR
Laport MS
Source :
Microbial pathogenesis [Microb Pathog] 2024 Oct; Vol. 195, pp. 106856. Date of Electronic Publication: 2024 Aug 15.
Publication Year :
2024

Abstract

Biofilm formation is a major health concern and studies have been pursued to find compounds able to prevent biofilm establishment and remove pre-existing biofilms. While biosurfactants (BS) have been well-known for possessing antibiofilm activities, bioemulsifiers (BE) are still scarcely explored for this purpose. The present study aimed to evaluate the bioemulsifying properties of cell-free supernatants produced by Bacillaceae and Vibrio strains isolated from marine sponges and investigate their antiadhesive and antibiofilm activities against different pathogenic Gram-positive and Gram-negative bacteria. The BE production by the marine strains was confirmed by the emulsion test, drop-collapsing, oil-displacement, cell hydrophobicity and hemolysis assays. Notably, Bacillus cereus 64BHI1101 displayed remarkable emulsifying activity and the ultrastructure analysis of its BE extract (BE64-1) revealed the presence of structures typically observed in macromolecules composed of polysaccharides and proteins. BE64-1 showed notable antiadhesive and antibiofilm activities against Staphylococcus aureus, with a reduction of adherence of up to 100 % and a dispersion of biofilm of 80 %, without affecting its growth. BE64-1 also showed inhibition of Staphylococcus epidermidis and Escherichia coli biofilm formation and adhesion. Thus, this study provides a starting point for exploring the antiadhesive and antibiofilm activities of BE from sponge-associated bacteria, which could serve as a valuable tool for future research to combat S. aureus biofilms.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Details

Language :
English
ISSN :
1096-1208
Volume :
195
Database :
MEDLINE
Journal :
Microbial pathogenesis
Publication Type :
Academic Journal
Accession number :
39153576
Full Text :
https://doi.org/10.1016/j.micpath.2024.106856