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Isolation, Purification of Phenolic Glycoside 1 from Moringa oleifera Seeds and Formulation of Its Liposome Delivery System.
- Source :
-
AAPS PharmSciTech [AAPS PharmSciTech] 2024 Aug 22; Vol. 25 (7), pp. 196. Date of Electronic Publication: 2024 Aug 22. - Publication Year :
- 2024
-
Abstract
- In this study, N, N '-bis {4- [(α-L- rhamnosyloxy) benzyl]} thiourea (PG-1), a phenolic glycoside compound was purified from Moringa seed. The PG-1 has attracted extensive attention due to its anti-cancer, antioxidant, anti-inflammatory and hypoglycemic properties. However, some of its physicochemical properties such as oral bioavailability has not been studied. Herein, a highly purified PG-1 was extracted and incorporated in multiple layered liposomes (PG-1-L) to avoid its burst release and enhance oral bioavailability. After appropriate characterization, it was discovered that the obtained PG-1-L was stable, homogeneous and well dispersed with the average particle size being 89.26 ± 0.23 nm. Importantly, the in vitro release and in vivo oral bioavailability of PG-1-L were significantly improved compared with PG-1. In addition, MTT results showed that compared with the free PG-1, PG-1-L displayed obvious inhibitory effect on the HepG2 cells, while the inhibitory effect on healthy non-malignant 3T6 and LO-2 cells was not significant, indicating that PG-1-L had high safety. In conclusion, PG-1-L can be used as a promising delivery system and an ideal novel approach to improve the oral bioavailability and anticancer activity of PG-1.<br /> (© 2024. The Author(s), under exclusive licence to American Association of Pharmaceutical Scientists.)
- Subjects :
- Humans
Animals
Hep G2 Cells
Particle Size
Drug Delivery Systems methods
Mice
Male
Rats
Administration, Oral
Chemistry, Pharmaceutical methods
Rats, Sprague-Dawley
Liposomes
Moringa oleifera chemistry
Seeds chemistry
Glycosides chemistry
Glycosides administration & dosage
Glycosides pharmacology
Glycosides isolation & purification
Biological Availability
Phenols administration & dosage
Phenols chemistry
Phenols isolation & purification
Phenols pharmacokinetics
Subjects
Details
- Language :
- English
- ISSN :
- 1530-9932
- Volume :
- 25
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- AAPS PharmSciTech
- Publication Type :
- Academic Journal
- Accession number :
- 39174848
- Full Text :
- https://doi.org/10.1208/s12249-024-02911-w