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Identification of two novel B-cell epitopes located on the spike protein of swine acute diarrhea syndrome coronavirus.
- Source :
-
International journal of biological macromolecules [Int J Biol Macromol] 2024 Oct; Vol. 278 (Pt 4), pp. 135049. Date of Electronic Publication: 2024 Aug 23. - Publication Year :
- 2024
-
Abstract
- Swine acute diarrhea syndrome coronavirus (SADS-CoV) is an emerging alpha-coronavirus that causes diarrhea in piglets and results in serious economic losses. During SADS-CoV infection, the spike protein (S) serves as a crucial structural component of the virion, interacting with receptors and eliciting the production of neutralizing antibodies. Due to the potential risk of zoonotic transmission of SADS-CoV, the identification and screening of epitopes on the S glycoproteins will be crucial for development of sensitive and specific diagnostic tools. In this study, we immunized BALB/c mice with recombinant SADS-CoV S trimer protein and generated two S1-specific monoclonal antibodies (mAbs): 8D6 and 6E9, which recognized different linear B-cell epitopes. The minimal fragment recognized by mAb 8D6 was mapped to <superscript>311</superscript> NPDQRD <superscript>316</superscript> , the minimal fragment recognized by mAb 6E9 was mapped to <superscript>492</superscript> ARFVDRL <superscript>498</superscript> . Homology analysis of the regions corresponding to 13 typical strains of different SADS-CoV subtypes showed high conservation of these two epitopes. These findings contribute to a deeper understanding of the structure of the SADS-CoV S protein, which is valuable for vaccine design and holds potential for developing diagnostic methods to detect SADS-CoV.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Mice
Swine
Epitope Mapping
Coronavirus Infections immunology
Coronavirus Infections diagnosis
Coronavirus Infections virology
Amino Acid Sequence
Antibodies, Viral immunology
Antibodies, Neutralizing immunology
Spike Glycoprotein, Coronavirus immunology
Spike Glycoprotein, Coronavirus chemistry
Epitopes, B-Lymphocyte immunology
Epitopes, B-Lymphocyte chemistry
Mice, Inbred BALB C
Antibodies, Monoclonal immunology
Alphacoronavirus immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0003
- Volume :
- 278
- Issue :
- Pt 4
- Database :
- MEDLINE
- Journal :
- International journal of biological macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 39182883
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2024.135049