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Genome-Wide Identification, Expression, and Protein Analysis of CKX and IPT Gene Families in Radish ( Raphanus sativus L.) Reveal Their Involvement in Clubroot Resistance.
- Source :
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International journal of molecular sciences [Int J Mol Sci] 2024 Aug 17; Vol. 25 (16). Date of Electronic Publication: 2024 Aug 17. - Publication Year :
- 2024
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Abstract
- Cytokinins (CKs) are a group of phytohormones that are involved in plant growth, development, and disease resistance. The isopentenyl transferase (IPT) and cytokinin oxidase/dehydrogenase (CKX) families comprise key enzymes controlling CK biosynthesis and degradation. However, an integrated analysis of these two gene families in radish has not yet been explored. In this study, 13 RsIPT and 12 RsCKX genes were identified and characterized, most of which had four copies in Brassica napus and two copies in radish and other diploid Brassica species. Promoter analysis indicated that the genes contained at least one phytohormone or defense and stress responsiveness cis-acting element. RsIPTs and RsCKXs were expanded through segmental duplication. Moreover, strong purifying selection drove the evolution of the two gene families. The expression of the RsIPT and RsCKX genes distinctly showed diversity in different tissues and developmental stages of the root. Expression profiling showed that RsCKX1-1/1-2/1-3 was significantly upregulated in club-resistant materials during primary infection, suggesting their vital function in clubroot resistance. The interaction network of CKX proteins with similar 3D structures also reflected the important role of RsCKX genes in disease resistance. This study provides a foundation for further functional study on the IPT and CKX genes for clubroot resistance improvement in Raphanus .
- Subjects :
- Alkyl and Aryl Transferases genetics
Alkyl and Aryl Transferases metabolism
Phylogeny
Plant Roots genetics
Plant Roots metabolism
Promoter Regions, Genetic
Gene Expression Profiling
Raphanus genetics
Gene Expression Regulation, Plant
Disease Resistance genetics
Plant Proteins genetics
Plant Proteins metabolism
Oxidoreductases genetics
Oxidoreductases metabolism
Multigene Family
Plant Diseases genetics
Plant Diseases parasitology
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 25
- Issue :
- 16
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 39201660
- Full Text :
- https://doi.org/10.3390/ijms25168974