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Methylated DNA Markers in Voided Urine for the Identification of Clinically Significant Prostate Cancer.
- Source :
-
Life (Basel, Switzerland) [Life (Basel)] 2024 Aug 18; Vol. 14 (8). Date of Electronic Publication: 2024 Aug 18. - Publication Year :
- 2024
-
Abstract
- Introduction: Non-invasive assays are needed to better discriminate patients with prostate cancer (PCa) to avoid over-treatment of indolent disease. We analyzed 14 methylated DNA markers (MDMs) from urine samples of patients with biopsy-proven PCa relative to healthy controls and further studied discrimination of clinically significant PCa (csPCa) from healthy controls and Gleason 6 cancers.<br />Methods: To evaluate the panel, urine from 24 healthy male volunteers with no clinical suspicion for PCa and 24 men with biopsy-confirmed disease across all Gleason scores was collected. Blinded to clinical status, DNA from the supernatant was analyzed for methylation signal within specific DNA sequences across 14 genes ( HES5 , ZNF655 , ITPRIPL1 , MAX.chr3.6187 , SLCO3A1 , CHST11 , SERPINB9 , WNT3A , KCNB2 , GAS6 , AKR1B1 , MAX.chr3.8028 , GRASP , ST6GALNAC2 ) by target enrichment long-probe quantitative-amplified signal assays.<br />Results: Utilizing an overall specificity cut-off of 100% for discriminating normal controls from PCa cases across the MDM panel resulted in 71% sensitivity (95% CI: 49-87%) for PCa detection (4/7 Gleason 6, 8/12 Gleason 7, 5/5 Gleason 8+) and 76% (50-92%) for csPCa (Gleason ≥ 7). At 100% specificity for controls and Gleason 6 patients combined, MDM panel sensitivity was 59% (33-81%) for csPCa (5/12 Gleason 7, 5/5 Gleason 8+).<br />Conclusions: MDMs assayed in urine offer high sensitivity and specificity for detection of clinically significant prostate cancer. Prospective evaluation is necessary to estimate discrimination of patients as first-line screening and as an adjunct to prostate-specific antigen (PSA) testing.
Details
- Language :
- English
- ISSN :
- 2075-1729
- Volume :
- 14
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Life (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 39202766
- Full Text :
- https://doi.org/10.3390/life14081024