An enzyme-linked immunosorbent assay for autoantibodies against the nuclear protein Scl-70.

Authors :: Geisler C
Høier-Madsen M
Source :: Journal of immunological methods [J Immunol Methods] 1985 Jun 25; Vol. 80 (2), pp. 211-9.
Publication Year :: 1985
Abstract: This paper describes the development of an enzyme-linked immunosorbent assay (ELISA) for the detection and quantitation of autoantibodies against the nuclear protein Scl-70. The isolation of Scl-70 from rat livers and the conditions for the ELISA are described. Compared with the already established double diffusion in gel for detection of anti-Scl-70 antibodies this ELISA has advantages.

Subjects :: Absorption
Animals
Antigens, Nuclear
Autoantigens isolation & purification
Collodion
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Fluorescent Antibody Technique
Immunodiffusion
Lupus Erythematosus, Systemic immunology
Nucleoproteins isolation & purification
Paper
Rats
Antigens immunology
Autoantibodies analysis
Autoantigens immunology
Nucleoproteins immunology
Scleroderma, Systemic immunology

Full Text Access

Tools