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An enzyme-linked immunosorbent assay for autoantibodies against the nuclear protein Scl-70.
- Source :
-
Journal of immunological methods [J Immunol Methods] 1985 Jun 25; Vol. 80 (2), pp. 211-9. - Publication Year :
- 1985
-
Abstract
- This paper describes the development of an enzyme-linked immunosorbent assay (ELISA) for the detection and quantitation of autoantibodies against the nuclear protein Scl-70. The isolation of Scl-70 from rat livers and the conditions for the ELISA are described. Compared with the already established double diffusion in gel for detection of anti-Scl-70 antibodies this ELISA has advantages.
- Subjects :
- Absorption
Animals
Antigens, Nuclear
Autoantigens isolation & purification
Collodion
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Fluorescent Antibody Technique
Immunodiffusion
Lupus Erythematosus, Systemic immunology
Nucleoproteins isolation & purification
Paper
Rats
Antigens immunology
Autoantibodies analysis
Autoantigens immunology
Nucleoproteins immunology
Scleroderma, Systemic immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1759
- Volume :
- 80
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of immunological methods
- Publication Type :
- Academic Journal
- Accession number :
- 3925022
- Full Text :
- https://doi.org/10.1016/0022-1759(85)90022-5