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Can Alpha-Pinene Prevent Methotrexate-Induced Cardiac and Hepatic Damage?
- Source :
-
Physiological research [Physiol Res] 2024 Aug 31; Vol. 73 (4), pp. 621-631. - Publication Year :
- 2024
-
Abstract
- The effects of alpha-pinene (AP), a monoterpenoid, known for its antioxidant, anti-inflammatory, and anti-apoptotic properties, on methotrexate (MTX)-induced cardiac and hepatic damage were investigated in this study. Male Sprague-Dawley rats were divided into Control, Vehicle, AP, MTX, and AP+MTX groups (n=7). AP (50 mg/kg/day, 14 days) was applied subcutaneously in the AP and AP+MTX groups. MTX (20 mg/kg) was injected three days before sacrification. Serum CK-MB, troponin T, ALT, and AST levels, as well as cardiac and hepatic MDA, GSH, caspase-3, and p53 levels, were measured by ELISA. Histological changes in tissues were evaluated by scoring in terms of tissue damage and cellular degeneration parameters after hematoxylin-eosin staining. MTX caused significant increase in serum CK-MB, troponin T, ALT, and AST levels, hepatic and cardiac lipid peroxidation, GSH depletion, and caspase-3 level. However, tissue levels of p53 did not change significantly. MTX-induced histological deterioration was observed in both tissues. These MTX-induced changes were significantly reduced in the AP+MTX group. Present results show that MTX-induced cardiac and hepatic damage is prevented by AP pretreatment. This protection can be attributed to the antioxidant and anti-apoptotic properties of AP. Considering the importance of MTX in cancer treatment, AP appears to have highly promising potential as a cardioprotective and hepatoprotective agent in anti-tumoral therapy. Key words: MDA, GSH, Caspase-3, p53, Oxidative stress, Apoptosis.
- Subjects :
- Animals
Male
Rats
Chemical and Drug Induced Liver Injury prevention & control
Chemical and Drug Induced Liver Injury pathology
Chemical and Drug Induced Liver Injury metabolism
Oxidative Stress drug effects
Antioxidants pharmacology
Liver drug effects
Liver pathology
Liver metabolism
Myocardium pathology
Myocardium metabolism
Monoterpenes pharmacology
Monoterpenes therapeutic use
Methotrexate toxicity
Rats, Sprague-Dawley
Bicyclic Monoterpenes pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1802-9973
- Volume :
- 73
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Physiological research
- Publication Type :
- Academic Journal
- Accession number :
- 39264082