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Universal sulfatase-based chemiluminescence biosensing platform: Validation via AFP detection in clinical blood samples.
- Source :
-
Biosensors & bioelectronics [Biosens Bioelectron] 2024 Sep 10; Vol. 267, pp. 116771. Date of Electronic Publication: 2024 Sep 10. - Publication Year :
- 2024
- Publisher :
- Ahead of Print
-
Abstract
- Enzyme-catalyzed chemiluminescence has been widely used in the field of biomedicine, especially in the test kit for various biomarkers. However, the currently reported enzyme-catalyzed chemiluminescence systems suffered from the addition of oxidizing substances, short emission wavelength, and susceptibility to interference by autofluorescence. In this paper, a universal sulfatase-based chemiluminescence system with NIR was developed, in which the designed substrate QM-CF could be transformed into 1,2-dioxetane derivate in the presence of sulfatase and oxygen. This system exhibited long emission wavelengths and CL half-time, a high signal-noise ratio, and without other additives. Importantly, the sulfatase-based chemiluminescence enzyme-linked immunoassay platform was successfully constructed and could be generally applied to detect biomarkers. As a proof of concept, the sulfatase-labeled AFP antibody and substate QM-CF were conveniently suitable for commercial AFP test kits, leading to satisfactory detection results of AFP in clinical blood samples.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier B.V. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1873-4235
- Volume :
- 267
- Database :
- MEDLINE
- Journal :
- Biosensors & bioelectronics
- Publication Type :
- Academic Journal
- Accession number :
- 39265427
- Full Text :
- https://doi.org/10.1016/j.bios.2024.116771