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Optimizing the Therapeutic Index of sdAb-Based Radiopharmaceuticals Using Pretargeting.
- Source :
-
Journal of nuclear medicine : official publication, Society of Nuclear Medicine [J Nucl Med] 2024 Oct 01; Vol. 65 (10), pp. 1564-1570. Date of Electronic Publication: 2024 Oct 01. - Publication Year :
- 2024
-
Abstract
- Single-domain antibodies (sdAbs) demonstrate favorable pharmacokinetic profiles for molecular imaging applications. However, their renal excretion and retention are obstacles for applications in targeted radionuclide therapy (TRT). Methods: Using a click-chemistry-based pretargeting approach, we aimed to reduce kidney retention of a fibroblast activation protein α (FAP)-targeted sdAb, 4AH29, for <superscript>177</superscript> Lu-TRT. Key pretargeting parameters (sdAb-injected mass and lag time) were optimized in healthy mice and U87MG (FAP <superscript>+</superscript> ) xenografts. A TRT study in a pancreatic ductal adenocarcinoma (PDAC) patient-derived xenograft (PDX) model was performed as a pilot study for sdAb-based pretargeting applications. Results: Modification of 4AH29 with trans -cyclooctene (TCO) moieties did not modify the sdAb pharmacokinetic profile. A 200-µg injected mass of 4AH29-TCO and an 8-h lag time for the injection of [ <superscript>177</superscript> Lu]Lu-DOTA-PEG <subscript>7</subscript> -tetrazine resulted in the highest kidney therapeutic index (2.0 ± 0.4), which was 5-fold higher than that of [ <superscript>177</superscript> Lu]Lu-DOTA-4AH29 (0.4 ± 0.1). FAP expression in the tumor microenvironment was validated in a PDAC PDX model with both immunohistochemistry and PET/CT imaging. Mice treated with the pretargeting high-activity approach (4AH29-TCO + [ <superscript>177</superscript> Lu]Lu-DOTA-PEG <subscript>7</subscript> -tetrazine; 3 × 88 MBq, 1 injection per week for 3 wk) demonstrated prolonged survival compared with the vehicle control and conventionally treated ([ <superscript>177</superscript> Lu]Lu-DOTA-4AH29; 3 × 37 MBq, 1 injection per week for 3 wk) mice. Mesangial expansion was reported in 7 of 10 mice in the conventional cohort, suggesting treatment-related kidney morphologic changes, but was not observed in the pretargeting cohort. Conclusion: This study validates pretargeting to mitigate sdAbs' kidney retention with no observation of morphologic changes on therapy regimen at early time points. Clinical translation of click-chemistry-based pre-TRT is warranted on the basis of its ability to alleviate toxicities related to biovectors' intrinsic pharmacokinetic profiles. The absence of representative animal models with extensive stroma and high FAP expression on cancer-associated fibroblasts led to a low mean tumor-absorbed dose even with high injected activity and consequently to modest survival benefit in this PDAC PDX.<br /> (© 2024 by the Society of Nuclear Medicine and Molecular Imaging.)
- Subjects :
- Animals
Mice
Humans
Cell Line, Tumor
Tissue Distribution
Female
Endopeptidases
Pancreatic Neoplasms radiotherapy
Pancreatic Neoplasms diagnostic imaging
Carcinoma, Pancreatic Ductal radiotherapy
Carcinoma, Pancreatic Ductal diagnostic imaging
Kidney diagnostic imaging
Membrane Proteins
Radiopharmaceuticals therapeutic use
Radiopharmaceuticals pharmacokinetics
Single-Domain Antibodies therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 1535-5667
- Volume :
- 65
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of nuclear medicine : official publication, Society of Nuclear Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 39266288
- Full Text :
- https://doi.org/10.2967/jnumed.124.267624