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A novel interaction between RNA m 6 A methyltransferase METTL3 and RREB1.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2024 Nov 12; Vol. 733, pp. 150668. Date of Electronic Publication: 2024 Sep 10. - Publication Year :
- 2024
-
Abstract
- Regulation of gene expression is achieved through the modulation of regulatory inputs both pre- and post-transcriptionally. Methyltransferase-like 3 (METTL3) is a key player in pre-mRNA processing, actively catalyzing N6-methyladenosine (m6A). Among the most enriched mRNA targets of METTL3 is the Ras Responsive Element Binding Protein 1 (RREB1), a transcription factor which functions to govern cell fate, proliferation and DNA repair. Here, we show a novel interaction between METTL3 and RREB1. Further examination of this interaction indicates that METTL3's N-terminus is the primary interacting domain. Our findings uncover a novel interacting partner of METTL3, providing further insights into METTL3's regulatory network.<br />Competing Interests: Declaration of competing interest R.C.R. was supported by the National Eye Institute (NEI) (R01EY030989), Research to Prevent Blindness (RPB), A. Alfred Taubman Medical Research Institute, the Beatrice and Reymont Paul Foundation, March Hoops to Beat Blindness, and Leonard G. Miller Endowed Professorship and Ophthalmic Research Fund at the Kellogg Eye Center. Additional support for this research was provided by Grossman, Elaine Sandman, Marek and Maria Spatz (endowed fund), Greenspon, Dunn, Avers, Boustikakis, Sweiden, and Terauchi research funds. C.W.R was supported by MSTP (T32GM007863). All other authors have no competing interests to declare.<br /> (Copyright © 2024 Elsevier Inc. All rights reserved.)
- Subjects :
- Humans
Adenosine analogs & derivatives
Adenosine metabolism
Adenosine genetics
DNA-Binding Proteins metabolism
DNA-Binding Proteins genetics
DNA-Binding Proteins chemistry
HEK293 Cells
Protein Binding
Methyltransferases metabolism
Methyltransferases chemistry
Methyltransferases genetics
Transcription Factors metabolism
Transcription Factors genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 733
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 39278095
- Full Text :
- https://doi.org/10.1016/j.bbrc.2024.150668