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A versatile fluorescent probe for hydrogen peroxide in serotonergic neurons of living brains of mice with depression.
- Source :
-
Journal of materials chemistry. B [J Mater Chem B] 2024 Oct 17; Vol. 12 (40), pp. 10241-10247. Date of Electronic Publication: 2024 Oct 17. - Publication Year :
- 2024
-
Abstract
- Depression, a prevalent mental illness, is intricately linked with the neurotransmitters in the brain, while serotonin as a crucial regulator of mood, energy levels, and memory, has been implicated in depression. So, the release of serotonin by serotonergic neurons plays a significant role in the development of depression. Notably, the foremost marker of oxidative stress, hydrogen peroxide (H <subscript>2</subscript> O <subscript>2</subscript> ), can interfere with the functioning of serotonergic neurons and potentially contribute to depression. Investigating the impact of H <subscript>2</subscript> O <subscript>2</subscript> on serotonergic neurons could offer valuable insights into the mechanisms underlying depression. However, there have been no effective tools for selectively imaging H <subscript>2</subscript> O <subscript>2</subscript> in these neurons so far. To address this gap, we created a small molecular fluorescent probe, PF-H2O2, designed specifically for imaging H <subscript>2</subscript> O <subscript>2</subscript> in serotonergic neurons under oxidative stress. PF-H2O2 exerts excellent serotonergic neuron-targetability and notable selectivity for H <subscript>2</subscript> O <subscript>2</subscript> . Furthermore, we discovered increased H <subscript>2</subscript> O <subscript>2</subscript> in serotonergic neurons of mice with depressive symptoms. Altogether, this endeavour unveils a pioneering tool for exploring pathophysiology linked to serotonergic neuronal dysfunction.
- Subjects :
- Animals
Mice
Brain metabolism
Oxidative Stress drug effects
Optical Imaging
Mice, Inbred C57BL
Serotonin metabolism
Serotonin analysis
Molecular Structure
Hydrogen Peroxide metabolism
Fluorescent Dyes chemistry
Fluorescent Dyes chemical synthesis
Depression metabolism
Serotonergic Neurons metabolism
Serotonergic Neurons chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 2050-7518
- Volume :
- 12
- Issue :
- 40
- Database :
- MEDLINE
- Journal :
- Journal of materials chemistry. B
- Publication Type :
- Academic Journal
- Accession number :
- 39282850
- Full Text :
- https://doi.org/10.1039/d4tb01828a