Anatomical and Micro-CT measurement analysis of ocular volume and intraocular volume in adult Bama Miniature pigs, New Zealand rabbits, and Sprague-Dawley rats.

Aim: Utilizing a combination of micro-computed tomography (micro-CT) and anatomical techniques for the volumetric assessment of the eyeball and its constituents in Bama Miniature Pigs, New Zealand rabbits, and Sprague-Dawley(SD) rats.
Method: Six Bama Miniature pigs, New Zealand rabbits, and SD rats were enrolled in the study. Micro-CT and gross volumetric estimation of ocular volume were employed to acquire data on ocular volume, anterior chamber volume, lens volume, and vitreous cavity volume for each eye.
Results: The eyeball volume of pigs ranges from approximately 5.36 ± 0.27 to 5.55 ± 0.28 ml, the lens volume from approximately 0.33 ± 0.02 to 0.37 ± 0.06 ml, the anterior chamber volume from approximately 0.19 ± 0.05 to 0.28 ± 0.04 ml, and the vitreous volume is approximately 3.20 ± 0.18 ml. For rabbits, the eye volume, lens volume, anterior chamber volume, and vitreous volume range from approximately 3.02 ± 0.24 to 3.04 ± 0.24 ml, 0.41 ± 0.02 to 0.44 ± 0.02 ml, 0.23 ± 0.04 to 0.26 ± 0.05 ml, and 1.54 ± 0.14 ml, respectively. In SD rats, the volumes are 0.14 ± 0.02 to 0.15 ± 0.01 ml for the eyeball, 0.03 ± 0.00 to 0.03 ± 0.00 ml for the lens, 0.01 ± 0.00 to 0.01 ± 0.01 ml for the anterior chamber, and 0.04 ± 0.01 ml for the vitreous volume.
Conclusion: The integration of micro-CT and gross volumetric estimation of ocular volume proves effective in determining the eyeball volume in Bama Miniature Pigs, New Zealand rabbits, and SD rats. Understanding the volume distinctions within the eyeballs and their components among these experimental animals can lay the groundwork for ophthalmology-related drug research.
Competing Interests: NO authors have competing interests.
(Copyright: © 2024 Wu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)