Increasing intracellular dNTP levels improves prime editing efficiency.

Authors :: Liu P
Ponnienselvan K
Nyalile T
Oikemus S
Joynt AT
Iyer S
Kelly K
Guo D
Kyawe PP
Vanderleeden E
Redick SD
Huang L
Chen Z
Lee JM
Schiffer CA
Harlan DM
Wang JP
Emerson CP Jr
Lawson ND
Watts JK
Sontheimer EJ
Luban J
Wolfe SA
Source :: Nature biotechnology [Nat Biotechnol] 2024 Sep 25. Date of Electronic Publication: 2024 Sep 25.
Publication Year :: 2024
Publisher :: Ahead of Print
Abstract: In primary cell types, intracellular deoxynucleotide triphosphate (dNTP) levels are tightly regulated in a cell cycle-dependent manner. We report that prime editing efficiency is increased by mutations that improve the enzymatic properties of Moloney murine leukemia virus reverse transcriptase and treatments that increase intracellular dNTP levels. In combination, these modifications produce substantial increases in precise editing rates.<br /> (© 2024. The Author(s), under exclusive licence to Springer Nature America, Inc.)