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In Situ Synthesis and Visualization of Membrane SNAP25 Nano-Organization.
- Source :
-
Langmuir : the ACS journal of surfaces and colloids [Langmuir] 2024 Oct 08; Vol. 40 (40), pp. 20977-20985. Date of Electronic Publication: 2024 Sep 27. - Publication Year :
- 2024
-
Abstract
- Cryo-electron tomography (cryo-ET) can provide insights into the structure and states of natural membrane environments to explore the role of SNARE proteins at membrane fusion and understand the relationship between their subcellular localization/formation and action mechanism. Nevertheless, the identification of individual molecules in crowded and low signal-to-noise ratio membrane environments remains a significant challenge. In this study, cryo-ET is employed to image near-physiological state 293T cell membranes, specifically utilizing in situ synthesized gold nanoparticles (AuNPs) bound with cysteine-rich protein tags to single-molecularly labeled synaptosomal-associated protein 25 (SNAP25) on the membrane surface. The high-resolution images reveal that SNAP25 is predominantly located in regions of high molecular density within the cell membrane and aggregates into smaller clusters, which may increase the fusion efficiency. Remarkably, a zigzag arrangement of SNAP25 is observed on the cell membrane. These findings provide valuable insights into the functional mechanisms of SNARE proteins.
Details
- Language :
- English
- ISSN :
- 1520-5827
- Volume :
- 40
- Issue :
- 40
- Database :
- MEDLINE
- Journal :
- Langmuir : the ACS journal of surfaces and colloids
- Publication Type :
- Academic Journal
- Accession number :
- 39330215
- Full Text :
- https://doi.org/10.1021/acs.langmuir.4c02231