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Lacticaseibacillus paracsei HY7207 Alleviates Hepatic Steatosis, Inflammation, and Liver Fibrosis in Mice with Non-Alcoholic Fatty Liver Disease.
- Source :
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International journal of molecular sciences [Int J Mol Sci] 2024 Sep 12; Vol. 25 (18). Date of Electronic Publication: 2024 Sep 12. - Publication Year :
- 2024
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Abstract
- Non-alcoholic fatty acid disease (NAFLD) is caused by a build-up of fat in the liver, inducing local inflammation and fibrosis. We evaluated the effects of probiotic lactic acid-generating bacteria (LAB) derived from a traditional fermented beverage in a mouse model of NAFLD. The LAB isolated from this traditional Korean beverage were screened using the human hepatic cell line HepG2, and Lactocaseibacillus paracasei HY7207 (HY7207), which was the most effective inhibitor of fat accumulation, was selected for further study. HY7207 showed stable productivity in industrial-scale culture. Whole-genome sequencing of HY7207 revealed that the genome was 2.88 Mbp long, with 46.43% GC contents and 2778 predicted protein-coding DNA sequences (CDSs). HY7207 reduced the expression of lipogenesis and hepatic apoptosis-related genes in HepG2 cells treated with palmitic acid. Furthermore, the administration of 10 <superscript>9</superscript> CFU/kg/day of HY7207 for 8 weeks to mice fed an NAFLD-inducing diet improved their physiologic and serum biochemical parameters and ameliorated their hepatic steatosis. In addition, HY7207 reduced the hepatic expression of genes important for lipogenesis ( Srebp1c , Fasn , C / ebpa , Pparg , and Acaca ), inflammation ( Tnf , Il1b , and Ccl2 ), and fibrosis ( Col1a1 , Tgfb1 , and Timp1 ). Finally, HY7207 affected the expression of the apoptosis-related genes Bax (encoding Bcl2 associated X, an apoptosis regulator) and Bcl2 (encoding B-cell lymphoma protein 2) in the liver. These data suggest that HY7207 consumption ameliorates NAFLD in mice through effects on liver steatosis, inflammation, fibrosis, and hepatic apoptosis. Thus, L. paracasei HY7207 may be suitable for use as a functional food supplement for patients with NAFLD.
- Subjects :
- Animals
Humans
Mice
Hep G2 Cells
Lacticaseibacillus paracasei
Male
Probiotics pharmacology
Disease Models, Animal
Apoptosis drug effects
Mice, Inbred C57BL
Liver pathology
Liver metabolism
Liver drug effects
Lipogenesis genetics
Lipogenesis drug effects
Lacticaseibacillus
Non-alcoholic Fatty Liver Disease metabolism
Non-alcoholic Fatty Liver Disease pathology
Non-alcoholic Fatty Liver Disease drug therapy
Liver Cirrhosis metabolism
Liver Cirrhosis pathology
Liver Cirrhosis drug therapy
Inflammation pathology
Inflammation metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 25
- Issue :
- 18
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 39337360
- Full Text :
- https://doi.org/10.3390/ijms25189870