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Characterizing the impact of CPSF30 gene disruption on TuMV infection in Arabidopsis thaliana .
- Source :
-
GM crops & food [GM Crops Food] 2024 Dec 31; Vol. 15 (1), pp. 1-17. Date of Electronic Publication: 2024 Oct 01. - Publication Year :
- 2024
-
Abstract
- CPSF30, a key polyadenylation factor, also serves as an m <superscript>6</superscript> A reader, playing a crucial role in determining RNA fate post-transcription. While its homologs mammals are known to be vital for viral replication and immune evasion, the full scope of CPSF30 in plant, particular in viral regulation, remains less explored. Our study demonstrates that CPSF30 significantly facilitates the infection of turnip mosaic virus (TuMV) in Arabidopsis thaliana , as evidenced by infection experiments on the engineered cpsf30 mutant. Among the two isoforms, CPSF30-L, which were characterized with m <superscript>6</superscript> A binding activity, emerged as the primary isoform responding to TuMV infection. Analysis of m <superscript>6</superscript> A components revealed potential involvement of the m <superscript>6</superscript> A machinery in regulating TuMV infection. In contrast, CPSF30-S exhibited distinct subcellular localization, coalescing with P-body markers (AtDCP1 and AtDCP2) in cytoplasmic granules, suggesting divergent regulatory mechanisms between the isoforms. Furthermore, comprehensive mRNA-Seq and miRNA-Seq analysis of Col-0 and cpsf30 mutants revealed global transcriptional reprogramming, highlighting CPSF30's role in selectively modulating gene expression during TuMV infection. In conclusion, this research underscores CPSF30's critical role in the TuMV lifecycle and sets the stage for further exploration of its function in plant viral regulation.
- Subjects :
- Gene Expression Regulation, Plant genetics
Mutation genetics
Arabidopsis genetics
Arabidopsis virology
Arabidopsis Proteins genetics
Arabidopsis Proteins metabolism
Plant Diseases virology
Plant Diseases genetics
Cleavage And Polyadenylation Specificity Factor genetics
Cleavage And Polyadenylation Specificity Factor metabolism
Potyvirus physiology
Potyvirus pathogenicity
Subjects
Details
- Language :
- English
- ISSN :
- 2164-5701
- Volume :
- 15
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- GM crops & food
- Publication Type :
- Academic Journal
- Accession number :
- 39351907
- Full Text :
- https://doi.org/10.1080/21645698.2024.2403776