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Glucosyltransferase TeGSS from Thermosynechococcus elongatus produces an α-1,2-glucan.

Glucosyltransferase TeGSS from Thermosynechococcus elongatus produces an α-1,2-glucan.

Authors :
Qu X
An Q
Sayed H
Cui L
Mayo KH
Su J
Source :
International journal of biological macromolecules [Int J Biol Macromol] 2024 Nov; Vol. 280 (Pt 4), pp. 136152. Date of Electronic Publication: 2024 Sep 30.
Publication Year :
2024

Abstract

Here, we enzymatically produced a novel α-1,2-glucan, glucosylsucrose, that has a chemical structure significantly different from that of other glucans. This structural difference suggests its potential to modulate new physiological activities compared to known glucans. The enzyme TeGSS catalyzes the synthesis of this α-1,2-glucan from sucrose and UDP-glucose (UDPG). Using NMR spectroscopy, we elucidated the chemical structures of TeGSS-synthesized glucosylsucrose tri-, tetra-, and penta-saccharides in which the monosaccharide units are linked by α-1,2-glycosidic bonds. We also report the crystal structures of TeGSS co-crystallized with UDP and glucosylsucrose tri- and tetra-saccharides. Site-directed mutagenesis of residues in and around the TeGSS catalytic center has allowed us to propose a concerted S <subscript>N</subscript> i mechanism of action. Finally, we developed an enzyme-coupled reaction involving TeGSS and SuSyAc that allows production of UDPG for the synthesis of α-1,2-glucan.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier B.V. All rights reserved.)

Details

Language :
English
ISSN :
1879-0003
Volume :
280
Issue :
Pt 4
Database :
MEDLINE
Journal :
International journal of biological macromolecules
Publication Type :
Academic Journal
Accession number :
39357710
Full Text :
https://doi.org/10.1016/j.ijbiomac.2024.136152