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Cancer cell-specific PD-L1 expression is a predictor of poor outcome in patients with locally advanced oral cavity squamous cell carcinoma.
- Source :
-
Journal for immunotherapy of cancer [J Immunother Cancer] 2024 Oct 02; Vol. 12 (10). Date of Electronic Publication: 2024 Oct 02. - Publication Year :
- 2024
-
Abstract
- Background: Locally advanced oral cavity squamous cell carcinoma (OCSCC) presents a significant clinical challenge despite being partially responsive to standard treatment modalities. This study investigates the prognostic implications of programmed death-ligand 1 (PD-L1) expression in these tumors, focusing on its association with treatment outcomes and the immune microenvironment.<br />Methods: We assessed tumor-infiltrating lymphocytes (TILs) in 132 patients with OCSCC to evaluate their impact on survival. Multiplex immunohistochemistry staining for CD3, CD68, CD11c, PD-L1, and P40 was used to explore correlations with clinical outcomes in patients with early-stage (n=22) and locally advanced (n=36) OCSCC. These initial findings were validated through differential gene expression analysis, gene set enrichment, and immune cell deconvolution in a The Cancer Genome Atlas cohort of 163 locally advanced OCSCC tumors. Additionally, single-cell RNA sequencing (scRNA-seq) on a smaller cohort (n=10) further characterized the PD-L1 <superscript>hi</superscript> or PD-L1 <superscript>lo</superscript> cancer cells in these tumors.<br />Results: Elevated PD-L1 expression was associated with poor outcomes in patients with locally advanced OCSCC undergoing standard adjuvant therapy, irrespective of "hot" or "cold" classification based on TILs assessment. PD-L1 <superscript>hi</superscript> tumors exhibited an active immune response phenotype, enriched with M1 macrophages, CD8 <superscript>+</superscript> T cells and T regulatory cells in the tumor microenvironment. Notably, the negative impact of PD-L1 expression on outcomes was primarily attributed to its expression by cancer cells, rather than immune cells. Furthermore, scRNA-seq revealed that immune interactions were not essential for PD-L1 upregulation in cancer cells, instead, complex regulatory networks were involved. Additionally, PD-L1 <superscript>lo</superscript> locally advanced tumors exhibited more complex pathway enrichment and diverse T-cell populations compared with those in the early-stage.<br />Conclusion: Our findings underscore the prognostic significance of PD-L1 expression in locally advanced OCSCC, and unveil the complex interplay between PD-L1 expression, immune responses, and molecular pathways in the tumor microenvironment. This study provides insights that may inform future therapeutic strategies, including the possibility of tailored immunotherapeutic approaches for patients with PD-L1 <superscript>hi</superscript> locally advanced OCSCC.<br />Competing Interests: Competing interests: No, there are no competing interests.<br /> (© Author(s) (or their employer(s)) 2024. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)
- Subjects :
- Humans
Male
Female
Middle Aged
Aged
Prognosis
Biomarkers, Tumor metabolism
Carcinoma, Squamous Cell immunology
Carcinoma, Squamous Cell metabolism
Carcinoma, Squamous Cell pathology
Carcinoma, Squamous Cell genetics
Adult
Squamous Cell Carcinoma of Head and Neck immunology
Squamous Cell Carcinoma of Head and Neck metabolism
Squamous Cell Carcinoma of Head and Neck genetics
Squamous Cell Carcinoma of Head and Neck pathology
Squamous Cell Carcinoma of Head and Neck mortality
B7-H1 Antigen metabolism
Mouth Neoplasms pathology
Mouth Neoplasms immunology
Mouth Neoplasms metabolism
Mouth Neoplasms genetics
Tumor Microenvironment
Lymphocytes, Tumor-Infiltrating immunology
Lymphocytes, Tumor-Infiltrating metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2051-1426
- Volume :
- 12
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal for immunotherapy of cancer
- Publication Type :
- Academic Journal
- Accession number :
- 39357980
- Full Text :
- https://doi.org/10.1136/jitc-2024-009617