Targeted Photoactivatable Green-Emitting BODIPY Based on Directed Photooxidation-Induced Activation and its Application to Live Dynamic Super-Resolution Microscopy.

Authors :: Saladin L
Le Berruyer V
Bonnevial M
Didier P
Collot M
Source :: Chemistry (Weinheim an der Bergstrasse, Germany) [Chemistry] 2024 Oct 04, pp. e202403409. Date of Electronic Publication: 2024 Oct 04.
Publication Year :: 2024
Publisher :: Ahead of Print
Abstract: Photoactivatable fluorescent probes are valuable tools in bioimaging for tracking cells down to single molecules and for single molecule localization microscopy. For the latter application, green emitting dyes are in demand. We herein developed an efficient green-emitting photoactivatable furanyl-BODIPY (PFB) and we established a new mechanism of photoactivation called Directed Photooxidation Induced Activation (DPIA) where the furan is photo-oxidized in a directed manner by the singlet oxygen produced by the probe. The efficient photoconverter (93-fold fluorescence enhancement at 510 nm, 49% yield conversion) is functionalizable and allowed targeting of several subcellular structures and organelles, which were photoactivated in live cells. Finally, we demonstrated the potential of PFB in super-resolution imaging by performing PhotoActivated Localization Microscopy (PALM) in live cells.<br /> (© 2024 Wiley‐VCH GmbH.)

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