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Improving the sulfite-detection performance of a fluorescent probe via post-synthetic modification with a metal-organic framework.
- Source :
-
Journal of materials chemistry. B [J Mater Chem B] 2024 Oct 08. Date of Electronic Publication: 2024 Oct 08. - Publication Year :
- 2024
- Publisher :
- Ahead of Print
-
Abstract
- In this work, a post-synthetic modification strategy was attempted to improve the performance of the probe for sulfite detection. The assembled platform UiO-66-NH-DQA, which was acquired by anchoring the sulfite-response fluorescent probe DQA onto the surface of UiO-66-NH <subscript>2</subscript> via amide covalent bonds, exhibited enhanced fluorescence intensity and practical intracellular imaging capability. In spite of the structural similarity, as verified by characterization tests, the conversion rate of post-synthetic modification was calculated as 35%, equaling an approximate assembly ratio of 1 : 2 between UiO-66-NH <subscript>2</subscript> and DQA. Most significantly, conversion into UiO-66-NH-DQA led to a 5.6-fold enhancement in the reporting signal with a red shift of 20 nm. For sulfite detection, the linear range was 0-150 μM, with a limit of detection value of 0.025 μM. UiO-66-NH-DQA retained advantages including high stability (within pH 5.0-9.0), rapid response (within 15 min) and high selectivity. Based on low cytotoxicity and relatively rapid cellular uptake, UiO-66-NH-DQA achieved the imaging of both the exogenous and endogenous sulfite levels in living cells. In particular, its rapid cell-permeating capability was guaranteed during the modification. The post-synthetic modification strategy reported herein has potential for improving the practical properties of fluorescent monitoring materials.
Details
- Language :
- English
- ISSN :
- 2050-7518
- Database :
- MEDLINE
- Journal :
- Journal of materials chemistry. B
- Publication Type :
- Academic Journal
- Accession number :
- 39376166
- Full Text :
- https://doi.org/10.1039/d4tb01754d