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PirA- or PirB-binding nanobodies can protect whiteleg shrimp from the acute hepatopancreatic necrosis disease toxin.

Authors :
Cheung S
Loutet SA
Zaytsoff S
Van Petegem F
Tran LH
Abnousi H
Source :
Diseases of aquatic organisms [Dis Aquat Organ] 2024 Oct 10; Vol. 160, pp. 7-12. Date of Electronic Publication: 2024 Oct 10.
Publication Year :
2024

Abstract

Acute hepatopancreatic necrosis disease (AHPND) is a devastating shrimp disease caused by a binary toxin, PirAB, produced by Vibrio parahaemolyticus and other closely related bacteria. To address AHPND, over 300 unique single-domain antibodies (also known as nanobodies) derived from the VHH domains of Lama glama heavy-chain-only antibodies were raised against either PirA or PirB and characterized. Nanobodies were shortlisted based on their affinities for either PirA or PirB, their relative stability in intestinal fluids, and their ability to reduce PirAB-induced death in brine shrimp Artemia salina. From these data, a subset of nanobodies was tested for their ability to reduce AHPND in whiteleg shrimp Penaeus vannamei, and nanobodies targeting either PirA or PirB provided significant disease protection to whiteleg shrimp. These results show that nanobodies can be a new option for shrimp farmers to reduce or eliminate the impact of AHPND on their operations.

Details

Language :
English
ISSN :
0177-5103
Volume :
160
Database :
MEDLINE
Journal :
Diseases of aquatic organisms
Publication Type :
Academic Journal
Accession number :
39387475
Full Text :
https://doi.org/10.3354/dao03817