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miR-326 overexpression inhibits colorectal cancer cell growth and proteasome activity by targeting PNO1: unveiling a novel therapeutic intervention strategy.
- Source :
-
Scientific reports [Sci Rep] 2024 Oct 16; Vol. 14 (1), pp. 24284. Date of Electronic Publication: 2024 Oct 16. - Publication Year :
- 2024
-
Abstract
- Proteasome inhibition emerges as a promising strategy for cancer prevention. PNO1, pivotal for colorectal cancer (CRC) progression, is involved in proteasome assembly in Saccharomyces cerevisiae. Hence, we aimed to explore the role of PNO1 in proteasome assembly and its up- and down-streams in CRC. Here, we demonstrated that PNO1 knockdown suppressed CRC cells growth, proteasome activities and assembly, as well as CDKN1B/p27Kip1 (p27) degradation. Moreover, p27 knockdown partially attenuated the inhibition of HCT116 cells growth by PNO1 knockdown. The up-stream studies of PNO1 identified miR-326 as a candidate miRNA directly targeting to CDS-region of PNO1 and its overexpression significantly down-regulated PNO1 protein expression, resulting in suppression of cell growth, decrease of proteasome activities and assembly, as well as increasing the stability of p27 in CRC cells. These findings indicated that miR-326 overexpression can suppress CRC cell growth, acting as an endogenous proteasome inhibitor by targeting PNO1.<br /> (© 2024. The Author(s).)
- Subjects :
- Humans
HCT116 Cells
Cell Line, Tumor
Proteasome Endopeptidase Complex metabolism
Colorectal Neoplasms genetics
Colorectal Neoplasms pathology
Colorectal Neoplasms metabolism
MicroRNAs genetics
MicroRNAs metabolism
Cell Proliferation genetics
Cyclin-Dependent Kinase Inhibitor p27 metabolism
Cyclin-Dependent Kinase Inhibitor p27 genetics
Gene Expression Regulation, Neoplastic
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 14
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 39414903
- Full Text :
- https://doi.org/10.1038/s41598-024-75746-x