Back to Search
Start Over
Comparative transcriptome analysis to identify the important mRNA and lncRNA associated with salinity tolerance in alfalfa.
- Source :
-
PeerJ [PeerJ] 2024 Oct 16; Vol. 12, pp. e18236. Date of Electronic Publication: 2024 Oct 16 (Print Publication: 2024). - Publication Year :
- 2024
-
Abstract
- Salinity represents a fatal factor affecting the productivity of alfalfa. But the regulation of salinity tolerance via lncRNAs and mRNAs remains largely unclear within alfalfa. For evaluating salinity stress resistance-related lncRNAs and mRNAs within alfalfa, we analyzed root transcriptomics in two alfalfa varieties, GN5 (salinity-tolerant) and GN3 (salinity-sensitive), after treatments with NaCl at 0 and 150 mM. There were altogether 117,677 lncRNAs and 172,986 mRNAs detected, including 1,466 lncRNAs and 2,288 mRNAs with significant differential expression in GN5 <subscript>150</subscript> /GN5 <subscript>0</subscript> , GN3 <subscript>150</subscript> /GN3 <subscript>0</subscript> , GN5 <subscript>0</subscript> /GN3 <subscript>0</subscript> , and GN5 <subscript>150</subscript> /GN3 <subscript>150</subscript> . As revealed by GO as well as KEGG enrichment, some ionic and osmotic stress-associated genes, such as HPCA1-LRR , PP2C60 , PP2C71 , CRK1 , APX3 , HXK2 , BAG6 , and ARF1 , had up-regulated levels in GN5 compared with in GN3. In addition, NaCl treatment markedly decreased CNGC1 expression in GN5. According to co-expressed network analyses, six lncRNAs (TCONS&#95;00113549, TCONS&#95;00399794, TCONS&#95;00297228, TCONS&#95;00004647, TCONS&#95;00033214 and TCONS&#95;00285177) modulated 66 genes including ARF1 , BAG6 , PP2C71 , and CNGC1 in alfalfa roots, suggesting that these nine genes and six lncRNAs probably facilitated the different salinity resistance in GN5 vs . GN3. These results shed more lights on molecular mechanisms underlying genotype difference in salinity tolerance among alfalfas.<br />Competing Interests: The authors declare that they have no competing interests.<br /> (© 2024 Yang et al.)
- Subjects :
- Plant Roots genetics
Plant Roots drug effects
Transcriptome
Medicago sativa genetics
RNA, Long Noncoding genetics
RNA, Long Noncoding metabolism
Salt Tolerance genetics
RNA, Messenger genetics
RNA, Messenger metabolism
Gene Expression Profiling
Gene Expression Regulation, Plant drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 2167-8359
- Volume :
- 12
- Database :
- MEDLINE
- Journal :
- PeerJ
- Publication Type :
- Academic Journal
- Accession number :
- 39430557
- Full Text :
- https://doi.org/10.7717/peerj.18236