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Dynamic susceptibility contrast-enhanced MRI with USPIO in evaluating angiogenesis of the peri-infarction zones in subacute ischemic stroke in a permanent middle cerebral artery occlusion rat model.
- Source :
-
Acta radiologica (Stockholm, Sweden : 1987) [Acta Radiol] 2024 Dec; Vol. 65 (12), pp. 1529-1539. Date of Electronic Publication: 2024 Oct 24. - Publication Year :
- 2024
-
Abstract
- Background: Dynamic susceptibility contrast-enhanced magnetic resonance imaging (DSC-MRI) can reflect the angiogenesis of ischemic stroke.<br />Purpose: To investigate the value of DSC-MRI with ultrasmall superparamagnetic particles of iron oxides (USPIO) in evaluating angiogenesis in the peri-infarction zones in subacute ischemic stroke in a permanent middle cerebral artery occlusion (pMCAO) rat model.<br />Material and Methods: A total of 21 Sprague-Dawley rats were randomly divided into the pMCAO and sham operation groups. Every rat in each group underwent DSC-MRI with USPIO at 3, 5, and 7 days. DSC-MRI parameters of the relative cerebral blood volume (rCBV), relative cerebral blood flow (rCBF), relative mean transit time (rMTT), and relative time to peak (rTTP) were measured, calculated, and compared among the different times. Sequential correlations were analyzed among the histopathological indexes with the microvascular density (MVD) and percentage of vascular area (%VA), the serum factors with vascular endothelial growth factor (VEGF), vascular cell adhesion molecule 1 (VCAM-1), and perfusion parameters, respectively.<br />Results: The rCBV and rCBF in the peri-infarction area of pMCAO rats were significantly higher on day 7 than on day 3, whereas no significant changes in rMTT and rTTP were observed at 3, 5, and 7 days. Significantly positive correlations were found between rCBV and MVD, %VA, VEGF, VCAM-1, between rCBF and MVD, %VA, VEGF, and VCAM-1 at 3, 5, and 7 days in the pMCAO group.<br />Conclusion: The rCBV and rCBF deriving from USPIO-DSC may be potentially useful for evaluating the angiogenesis of the peri-infarction zones in the subacute phase of ischemic stroke.<br />Competing Interests: Declaration of conflicting interestsThe authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
- Subjects :
- Animals
Rats
Male
Dextrans
Ischemic Stroke diagnostic imaging
Neovascularization, Pathologic diagnostic imaging
Angiogenesis
Rats, Sprague-Dawley
Infarction, Middle Cerebral Artery diagnostic imaging
Contrast Media
Magnetic Resonance Imaging methods
Disease Models, Animal
Magnetite Nanoparticles
Subjects
Details
- Language :
- English
- ISSN :
- 1600-0455
- Volume :
- 65
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Acta radiologica (Stockholm, Sweden : 1987)
- Publication Type :
- Academic Journal
- Accession number :
- 39449316
- Full Text :
- https://doi.org/10.1177/02841851241290646