Back to Search
Start Over
Unveiling Novel Mechanism of CIDEB in Fatty Acid Synthesis Through ChIP-Seq and Functional Analysis in Dairy Goat.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2024 Oct 21; Vol. 25 (20). Date of Electronic Publication: 2024 Oct 21. - Publication Year :
- 2024
-
Abstract
- Goat milk is abundant in nutrients, particularly in milk fats, which confer health benefits to humans. Exploring the regulatory mechanism of fatty acid synthesis is highly important to understand milk composition manipulation. In this study, we used chromatin immunoprecipitation sequencing (ChIP-seq) on goat mammary glands at different lactation stages which revealed a novel lactation regulatory factor: cell death-inducing DFFA-like effector B (CIDEB). RT-qPCR results revealed that CIDEB was significantly upregulated during lactation in dairy goats. CIDEB overexpression significantly increased the expression levels of genes involved in fatty acid synthesis ( ACACA , SCD1 , p < 0.05; ELOVL6 , p < 0.01), lipid droplet formation ( XDH , p < 0.05), and triacylglycerol (TAG) synthesis ( DGAT1 , p < 0.05; GPAM , p < 0.01) in goat mammary epithelial cells (GMECs). The contents of lipid droplets, TAG, and cholesterol were increased ( p < 0.05) in CIDEB-overexpressing GMECs, and knockdown of CIDEB led to the opposite results. In addition, CIDEB knockdown significantly decreased the proportion of C16:0 and total C18:2. Luciferase reporter assays indicated that X-box binding protein 1 (XBP1) promoted CIDEB transcription via XBP1 binding sites located in the CIDEB promoter. Furthermore, CIDEB knockdown attenuated the stimulatory effect of XBP1 on lipid droplet accumulation. Collectively, these findings elucidate the critical regulatory roles of CIDEB in milk fat synthesis, thus providing new insights into improving the quality of goat milk.
- Subjects :
- Animals
Female
Chromatin Immunoprecipitation Sequencing methods
Milk metabolism
Epithelial Cells metabolism
Promoter Regions, Genetic
Apoptosis Regulatory Proteins genetics
Apoptosis Regulatory Proteins metabolism
Gene Expression Regulation
Triglycerides metabolism
Triglycerides biosynthesis
Lipid Droplets metabolism
Goats genetics
Goats metabolism
Fatty Acids metabolism
Fatty Acids biosynthesis
Lactation genetics
Mammary Glands, Animal metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 25
- Issue :
- 20
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 39457100
- Full Text :
- https://doi.org/10.3390/ijms252011318