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Ultrafast Evolution of Bacterial Antimicrobial Resistance by Picoliter-Scale Centrifugal Microfluidics.

Authors :
Xu T
Dai Y
Ge A
Chen X
Gong Y
Lam TH
Lee K
Han X
Ji Y
Shen W
Liu J
Sun L
Xu J
Ma B
Source :
Analytical chemistry [Anal Chem] 2024 Nov 26; Vol. 96 (47), pp. 18842-18851. Date of Electronic Publication: 2024 Nov 12.
Publication Year :
2024

Abstract

Experimental evolution is a powerful approach for scrutinizing and dissecting the development of antimicrobial resistance; nevertheless, it typically demands an extended duration to detect evolutionary changes. Here, a centrifugal microfluidics system is designed to accelerate the process. Through a simple step of on-chip centrifugation, a highly condensed bacterial matrix of ∼10 <superscript>12</superscript> cells/mL at the enrichment tip of the chip channel is derived, enabling bacteria encapsulated to survive in antimicrobial concentrations several times higher than the minimum inhibitory concentration (MIC) and rapidly develop resistance in the first 10 h. After 48 h of on-chip evolution, the E. coli strain demonstrated a 64 to 128-fold reduction in sensitivity to disinfectants (triclosan) as well as antibiotics (ciprofloxacin and amikacin), a rate substantially swifter compared to conventional continuous inoculation-based experimental evolution. The speed and simplicity of this microfluidic system suggest its broad application for uncovering resistance mechanisms and identifying targets of biocides and antibiotics.

Details

Language :
English
ISSN :
1520-6882
Volume :
96
Issue :
47
Database :
MEDLINE
Journal :
Analytical chemistry
Publication Type :
Academic Journal
Accession number :
39531253
Full Text :
https://doi.org/10.1021/acs.analchem.4c04482