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Whole genome sequencing of CRISPR/Cas9-engineered NF-κB reporter mice for validation and variant discovery.
- Source :
-
Scientific data [Sci Data] 2024 Nov 13; Vol. 11 (1), pp. 1225. Date of Electronic Publication: 2024 Nov 13. - Publication Year :
- 2024
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Abstract
- Targeted knockout, mutations, or knock-in of genomic DNA fragments in model organisms have been used widely for functional and cell-tracking studies. The desired genetic perturbation is often accomplished by recombination-based or CRISPR/Cas9-based genome engineering. For validating the intended genetic modification, a local region surrounding the targeted locus is typically examined based on enzymatic cleavage and consequent length patterns, e.g. in a Southern analysis. Despite its wide use, this approach is open to incomplete and ambiguous readouts. With decreasing costs of high-throughput sequencing, it is becoming feasible to consider a large-scale validation of a new strain after a targeted genetic perturbation. Here we describe a dataset of whole-genome sequences and the variant analysis results from four novel reporter mouse strains. This served to validate the strains and identified all the off-target effects on the genome, thereby increasing the genetic diversity of genomic sequences over those represented in the public databases for inbred mice.<br />Competing Interests: Competing interests The authors declare no competing interests.<br /> (© 2024. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.)
Details
- Language :
- English
- ISSN :
- 2052-4463
- Volume :
- 11
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific data
- Publication Type :
- Academic Journal
- Accession number :
- 39537647
- Full Text :
- https://doi.org/10.1038/s41597-024-04064-8