Ultrasonic-assisted plasma-activated water extraction of polysaccharide from Hemerocallis citrina Baroni: Structural characterization and antioxidant mechanism in vitro.

Natural polysaccharides derived from Hemerocallis citrina Baroni exhibit significant biological activity. To enhance extraction efficiency and antioxidant activity, ultrasonic-assised plasma-activated water (PAW) was utilized to extract polysaccharides from Hemerocallis citrina Baroni. PAW demonstrated enhanced permeability and diffusion capabilities due to a reduced percentage of fully hydrogen-bonded structures, along with optimal pH (3.4) and conductivity (157.20 μS/cm). Compared to other methods, PAW significantly improved the extraction efficiency of polysaccharides to 38.24 %. Hemerocallis citrina Baroni polysaccharides predominantly consisted of α-pyranose sugars with sugar uronic acids, and ultrasound-assisted PAW did not alter their major functional groups. The extracted Hemerocallis citrina Baroni polysaccharides exhibited a semi-rigid triple-helical structure. Importantly, these polysaccharides displayed strong antioxidant activity, with ABTS ⁺ , DPPH, and O ₂ ^- radical scavenging rates of 66.31 %, 65.98 %, and 47.73 % respectively. This study provides an effective method for extracting natural plant polysaccharides and offers valuable insights into their potential applications in the food industry and beyond.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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