The predictive and prognostic value of tumor-stromal ratio and tumor budding in the recurrence and outcome of patients with oral tongue squamous cell carcinoma.

Introduction: This study aimed to explore the predictive and prognostic value of tumor-stromal ratio (TSR) and tumor budding (TB) in the recurrence and outcome of patients with Oral tongue squamous cell carcinoma (OTSCC).
Methods: All patients with OTSCC who underwent glossectomy with or without neck dissection in a tertiary center between 2010 and 2020 were included. The pathology slides of all patients were reviewed by a consulting pathologist. All pathologic features including tumor size, tumor grade, tumor thickness, surgical margin status, lymphovascular- and perineural invasion, T-stage, number of dissected, TB, TSR, and involved, tumor budding and tumor-stromal ratio were defined, we categorized the tumors as stroma-rich (low TSR) and stroma-poor (high TSR); as well as TB as low (0-4 buds) and high (≥ 5 buds). The inter-observer reliability of TB and TSR was assessed using the Kappa statistics analysis.
Results: A total of 109 patients, with a median age of 62 (range 19-88) years, were included. There was a significant correlation between TSR and tumor budding with other adverse pathologic features and prognostic factors. In univariate analysis, both stromal-rich tumors and tumor budding were poor prognostic factors for disease-free survival (DFS) and overall survival (OS). However, on multivariate analysis, N-stage, adjuvant radiotherapy, tumor size, depth of invasion (DOI) and tumor budding emerged as independent prognostic factors for DFS. Likewise, N-stage, and DOI were recognized as independent prognostic factors for OS.
Conclusion: Both TSR and TB are important histopathological parameters strongly linked to other pathologic prognostic indicators. Additionally, they play key roles in predicting tumor behavior, recurrence, and patient survival.
Competing Interests: Declarations. Humanal approval statement: In accordance with the code of ethics of the World Medical Association (Declaration of Helsinki) for experiments involving humans, the study protocol was approved by the Research Ethics Committee of Shiraz University of Medical Sciences (IR.SUMS.DENTAL.REC.1402.013). Competing interest: The authors declare no competing interests. Consent to participate: Written consent was obtained from all patients enrolled in this retrospective study for diagnostic and treatment purposes. All patient information was kept confidential and anonymized to protect their privacy. The study was conducted in compliance with all relevant ethical guidelines and regulations. Conflict of interest: All authors declare that they have no conflicts of interest.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)