Ten-month comprehensive assessment of steroid hormones in the tributaries of Baiyun District, Guangzhou City, China: Spatiotemporal dynamics, source attribution, and environmental implications.

The occurrence of steroid hormones in small river ecosystems raises environmental alarms due to their limited dilution capacity, heightened susceptibility to diverse pollution sources, and their substantial contribution to the contamination of larger river systems. Here, we investigated the occurrence of 40 steroid hormones over 10 months in 10 first-order tributaries (n = 250) of Guangzhou City, China. The observed concentrations of Σsteroid hormones ranged from 30.5 to 450 ng/L (mean: 55.6 ± 35.4 ng/L). No substantial variation in steroid hormone concentrations was observed between the flood and dry seasons, reflecting an intricate balance of dilution dynamics, agricultural runoff, and wastewater releases. Further correlation analysis underscored wastewater discharge as a consistent source of steroid hormone occurrence, with spikes coinciding with concurrent fertilizer application and rainfall intervals. Steroid hormone concentrations displayed significant spatial variations. Correlation analyses connected steroid hormone levels to nutrients in tributaries and agricultural ditch water and land usage, highlighting the joint effect of runoff and various wastewater types on steroid hormone distribution. Interestingly, steroid hormone levels displayed minimal variation along the tributaries, suggesting uniform and continuous pollution sources. Source attribution analysis revealed that 51.7 % of steroid hormones originated from untreated domestic wastewater, followed by treated wastewater, livestock wastewater, and runoff. Notably, 92.0 % of the sampling sites registered at least one steroid hormone level exceeding the risk quotient threshold of 1, indicating widespread ecological hazards. Our research emphasizes the persistent and stable nature of steroid hormone-related risks across seasons and along the tributaries, highlighting the imperative for vigilant monitoring. We further advocate for intensified surveillance efforts during pivotal periods (e.g., fertilization periods and low rainfall intervals), to better address these environmental challenges.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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