Back to Search
Start Over
Single-cell RNA-seq reveals FGF12 as a prognostic biomarker in low-grade endometrial stromal sarcoma.
- Source :
-
Frontiers in immunology [Front Immunol] 2024 Nov 29; Vol. 15, pp. 1513076. Date of Electronic Publication: 2024 Nov 29 (Print Publication: 2024). - Publication Year :
- 2024
-
Abstract
- Background: Low-grade endometrial stromal sarcoma (LG-ESS) is a rare uterine malignancy characterized by its complex tumor microenvironment (TME) and high recurrence rates, posing challenges to accurate prognosis and effective treatment. Identifying prognostic biomarkers is essential for improving patient stratification and guiding therapeutic strategies.<br />Methods: Using single-cell transcriptome analysis combined with H&E and multiplex immunofluorescence staining, we identified a subpopulation of tumor cells in LG-ESS and further validated the association of this subpopulation and its characteristic genes with LG-ESS prognosis by molecular characterization and bulk transcriptome data.<br />Results: Our analysis reveals multiple cellular subpopulations within the tumor tissue, particularly a tumor cell subpopulation among them which is associated with poor prognosis. Originating from normal stromal fibroblasts, this subpopulation appears to play a crucial role in TME remodeling, smooth muscle cell behavior, and potentially in tumorigenesis and metastasis. Of particular interest in this subpopulation is the highly expressed FGF12 gene, which is significantly associated with a shortened survival in ESS, highlighting its potential as a prognostic biomarker.<br />Conclusion: Our study reveals the complexity of TME within the LG-ESS and highlights the role that tumor cell subpopulations play in disease progression and patient prognosis. The identification of FGF12 as a prognostic biomarker suggests a new approach for the personalized treatment and prognosis monitoring of patients.<br />Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision.<br /> (Copyright © 2024 Miao, Dong, Zhou, Thiel, Li, Cai, Yuan, Wang, Jin, Yang, Wang, Frey, Gaipl, Ma and Zhou.)
- Subjects :
- Humans
Female
Prognosis
Neoplasm Grading
Transcriptome
Gene Expression Regulation, Neoplastic
Gene Expression Profiling
Single-Cell Gene Expression Analysis
Biomarkers, Tumor genetics
Sarcoma, Endometrial Stromal genetics
Sarcoma, Endometrial Stromal pathology
Sarcoma, Endometrial Stromal metabolism
Sarcoma, Endometrial Stromal diagnosis
Single-Cell Analysis
Endometrial Neoplasms genetics
Endometrial Neoplasms mortality
Endometrial Neoplasms pathology
Endometrial Neoplasms diagnosis
RNA-Seq
Tumor Microenvironment genetics
Fibroblast Growth Factors genetics
Fibroblast Growth Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1664-3224
- Volume :
- 15
- Database :
- MEDLINE
- Journal :
- Frontiers in immunology
- Publication Type :
- Academic Journal
- Accession number :
- 39676856
- Full Text :
- https://doi.org/10.3389/fimmu.2024.1513076