Study on the antioxidant and antiosteoporotic activities of the oyster peptides prepared by ultrasound-assisted enzymatic hydrolysis.

In this study, the effects of ultrasound-assisted enzymatic hydrolysis on the production of antioxidant and antiosteoporotic peptides derived from oysters were investigated. Results showed that ultrasound-assisted enzymatic hydrolysis significantly enhanced the peptide content, free radical scavenging ability, and ferric reducing antioxidant power of total oyster protein hydrolysate (TOPH), with optimal results achieved at 200 W (TOPH-200). Correspondingly, ultrasound treatment at 200 W increased the exposure of hydrophobic regions, reduced α-helix content, and facilitated the generation of small molecular weight peptides in TOPH. In an H ₂ O ₂ -induced oxidative damage model of osteoblastic MC3T3-E1 cells, TOPH-200 significantly attenuated intracellular reactive oxygen species and improved mitochondrial membrane potential. Importantly, TOPH-200 effectively enhanced osteogenic cell proliferation, differentiation, and mineralization in H ₂ O ₂ -treated MC3T3-E1 cells. Additionally, two novel peptides, DSQLAPFRF and HFNPRL, were screened from the TOPH-200 using PeptideRanker and molecular docking. Further cell experiments indicated that both peptides exhibited potent antioxidant and antiosteoporotic activities in oxidatively damaged MC3T3-E1 cells. In summary, mild ultrasound-assisted enzymatic hydrolysis proved effective in producing bioactive peptides from oysters, and these newly identified peptides exhibit potential for osteoporosis prevention.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.)