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Isolation and characterization of Saprolegnia parasitica from cage-reared Pangasianodon hypophthalmus and its sensitivity to different antifungal compounds.

Authors :
Rathod SK
Das BK
Tandel RS
Chatterjee S
Das N
Tripathi G
Kumar S
Panda SK
Patil PK
Manna SK
Source :
Scientific reports [Sci Rep] 2024 Dec 28; Vol. 14 (1), pp. 30720. Date of Electronic Publication: 2024 Dec 28.
Publication Year :
2024

Abstract

Saprolegniasis is one of the most dangerous fungal diseases of fish, causing significant mortality in fish hatcheries and young ones. The present study aimed to isolate and characterize the causative fungus from fingerlings of Pangasianodon hypophthalmus cultured intensively in freshwater cages in Indian reservoirs and to determine minimum inhibitory concentrations of different antifungal compounds against the fungal hyphae and zoospores. The fungal isolates grown on potato dextrose agar showed an abundance of gemmae, elongated mycelia, non-septate hyphae, primary zoospores, mature zoosporangia with numerous zoospores, cysts with bundles of long hairs and were further identified as Saprolegnia parasitica following PCR amplification and sequencing of internal transcribed spacer region. S. parasitica showed temperature-sensitive optimum growth in a narrow window of 12-24 ℃, which might drive its experimental pathogenesis as well as natural infections in the winter months. In vitro sensitivity testing established negligible inhibitory activity of fluconazole, boric acid, sodium thiosulfate, and potassium permanganate while clotrimazole arrested the spore and hyphal growths at 2 mgL <superscript>-1</superscript> concentration suggesting potential of the imidazole antifungal in treating S. parasitica infection in fish. The present study will serve as the baseline information for developing therapeutic and management strategies for controlling saprolegniasis in the economically significant iridescent catfish.<br />Competing Interests: Declarations. Competing interests: The authors declare no competing interests.<br /> (© 2024. The Author(s).)

Details

Language :
English
ISSN :
2045-2322
Volume :
14
Issue :
1
Database :
MEDLINE
Journal :
Scientific reports
Publication Type :
Academic Journal
Accession number :
39730460
Full Text :
https://doi.org/10.1038/s41598-024-80075-0