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Bufalin Ameliorates Myocardial Ischemia/Reperfusion Injury by Suppressing Macrophage Pyroptosis via P62 Pathway.

Authors :
Li C
Ma Z
Wei X
Wang Y
Wu J
Li X
Sun X
Ding Z
Yang C
Zou Y
Source :
Journal of cardiovascular translational research [J Cardiovasc Transl Res] 2024 Dec 28. Date of Electronic Publication: 2024 Dec 28.
Publication Year :
2024
Publisher :
Ahead of Print

Abstract

Bufalin, which is isolated from toad venom, exerts positive effects on hearts under pathological circumstance. We aimed to investigate the effects and mechanisms of bufalin on myocardial I/R injury. In vivo, bufalin ameliorated myocardial I/R injury, which characteristics with better ejection function, decreased infarct size and less apoptosis. The levels of pyroptotic proteins were increased in I/R-treated macrophages and inflammatory cytokines expressed more in I/R-induced mouse, which could be attenuated by bufalin. Bufalin also reduced H/R-treated macrophage pyroptosis in vitro. Autophagic flux blockage and ROS accumulation were reduced by bufalin in impaired macrophages. Overexpression of p62 abrogated the anti-proptosis and anti-oxidative effects of bufalin. The levels of apoptosis related proteins were changed and TUNEL-positive ratio was raised in cardiomyocytes that received conditioned medium treatment with H/R-treated macrophages, while bufalin pretreatment could reduce apoptosis. These findings indicate that bufalin may attenuate myocardial I/R injury by suppressing macrophage pyroptosis via P62 pathway.<br />Competing Interests: Declarations. Ethics Approval: No human studies were carried out by the authors for this article. All the experiments complied with the guiding principles for the care and use of laboratory animals of Zhongshan Hospital, Fudan University and were approved by the Committee for Animal Experimentation. Disclosures: None. Conflict of Interest: All authors disclosed no relevant relationships.<br /> (© 2024. The Author(s).)

Details

Language :
English
ISSN :
1937-5395
Database :
MEDLINE
Journal :
Journal of cardiovascular translational research
Publication Type :
Academic Journal
Accession number :
39733202
Full Text :
https://doi.org/10.1007/s12265-024-10577-9