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Evaluation of colony-forming ability experiments using normal and DNA repair-deficient human fibroblast strains and an automatic colony counter.
- Source :
-
Cytometry [Cytometry] 1985 Mar; Vol. 6 (2), pp. 130-6. - Publication Year :
- 1985
-
Abstract
- An automated procedure for evaluation of colony-forming ability experiments has been developed using normal and xeroderma pigmentosum fibroblast strains. Technically this consists of an image analyzer equipped with a contrast-intensifying video camera, monitor, and a desk-top computer. Complex structures composed of overlapping colonies could be reduced to the constituent single colonies by special correction programs. Eighteen experiments were evaluated, and the D0 values for colony-forming ability were determined. A systematic but correctable deviation between visual and automated counting was found. The source of this deviation is described and a solution for correction is provided. The automatic procedure reduced the evaluating time by a factor of five to ten.
Details
- Language :
- English
- ISSN :
- 0196-4763
- Volume :
- 6
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Cytometry
- Publication Type :
- Academic Journal
- Accession number :
- 3979217
- Full Text :
- https://doi.org/10.1002/cyto.990060208