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Characterization of the chromophore of the third rhodopsin-like pigment of Halobacterium halobium and its photoproduct.
- Source :
-
Biophysical journal [Biophys J] 1985 May; Vol. 47 (5), pp. 721-4. - Publication Year :
- 1985
-
Abstract
- Halobacterium halobium contains at least three retinal-containing pigments: bacteriorhodopsin, halorhodopsin, and a third rhodopsin-like pigment (tR) absorbing at approximately 590 nm, tR590. Illumination of tR590 gives rise to a very long-lived blue absorbing photoproduct, tR370. Using high-performance liquid chromatography we show that the chromophore of tR590 is primarily all-trans retinal and its conversion by light to tR370 causes the chromophore to isomerize primarily to the 13-cis conformation. Irradiation of the tR370 gives rise to a transient photoproduct absorbing at approximately 520 nm that decays back to the initial pigment tR590. In addition to all-trans retinal, the apomembrane of tR can also combine with 13-cis retinal but not with the 9- or 11-cis isomers.
Details
- Language :
- English
- ISSN :
- 0006-3495
- Volume :
- 47
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Biophysical journal
- Publication Type :
- Academic Journal
- Accession number :
- 4016190
- Full Text :
- https://doi.org/10.1016/S0006-3495(85)83969-2