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Effects of probes of membrane potential on metabolism in synaptosomes.
- Source :
-
Biochimica et biophysica acta [Biochim Biophys Acta] 1985 Nov 22; Vol. 843 (1-2), pp. 20-4. - Publication Year :
- 1985
-
Abstract
- Effects of three probes for measuring membrane potential, tetraphenylphosphonium (TPP+), rhodamine 6G and 3,3'-dipropylthiocarbocyanine (diS-C3-(5)) on energy metabolism in synaptosomes were investigated. None of the three probes had any effect on lactate production in synaptosomes. TPP+ and rhodamine 6G did not inhibit the respiration of synaptosomes with pyruvate and succinate as exogenous substrate and were only weakly inhibitory with endogenous substrates. In contrast, diS-C3-(5) markedly inhibited the respiration of synaptosomes with glucose, pyruvate and endogenous substrates. All three probes reduced ATP content in synaptosomes and depolarized the membrane potential in synaptosomes with increasing concentrations of the probes. It is, therefore, preferable to estimate membrane potential with TPP+ or rhodamine 6G at their low concentrations where their effect on metabolism is negligible.
- Subjects :
- Adenosine Triphosphate metabolism
Animals
Benzothiazoles
Brain Chemistry drug effects
Glucose metabolism
In Vitro Techniques
Lactates biosynthesis
Oxygen Consumption drug effects
Pyruvates metabolism
Pyruvic Acid
Rats
Succinates metabolism
Succinic Acid
Synaptosomes drug effects
Carbocyanines pharmacology
Coloring Agents pharmacology
Membrane Potentials drug effects
Onium Compounds pharmacology
Organophosphorus Compounds pharmacology
Quinolines pharmacology
Rhodamines pharmacology
Synaptosomes metabolism
Xanthenes pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0006-3002
- Volume :
- 843
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Biochimica et biophysica acta
- Publication Type :
- Academic Journal
- Accession number :
- 4063387
- Full Text :
- https://doi.org/10.1016/0304-4165(85)90044-3