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D-glucaric acid and galactaric acid catabolism by Agrobacterium tumefaciens.
- Source :
-
Journal of bacteriology [J Bacteriol] 1970 Apr; Vol. 102 (1), pp. 85-96. - Publication Year :
- 1970
-
Abstract
- Cell-free extract (crude extract) of Agrobacterium tumefaciens grown on d-glucuronate or d-glucarate converts d-glucarate and galactarate to a mixture of 2-keto-3-deoxy- and 4-deoxy-5-keto-d-glucarate. These compounds are then converted by partially purified crude extract to an intermediate tentatively identified as 2,5-diketoadipate. The same enzyme preparation further decarboxylates this intermediate to alpha-ketoglutarate semialdehyde, which is subsequently oxidized in a nicotinamide adenine dinucleotide-dependent reaction to alpha-ketoglutaric acid. Since A. tumefaciens converts d-glucuronic acid to d-glucarate, a pathway from d-glucuronate to alpha-ketoglutarate in A. tumefaciens was determined.
- Subjects :
- Carbon Isotopes
Centrifugation
Chromatography, Paper
Chromatography, Thin Layer
Electrophoresis
Glucuronates metabolism
Hydro-Lyases metabolism
Ketoglutaric Acids biosynthesis
NAD pharmacology
Paper
Quaternary Ammonium Compounds
Rhizobium enzymology
Spectrophotometry
Sulfates
Ultraviolet Rays
Adipates metabolism
Rhizobium metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9193
- Volume :
- 102
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 4314480
- Full Text :
- https://doi.org/10.1128/jb.102.1.85-96.1970