Back to Search
Start Over
Depression of guanosine diphosphate-mannose pyrophosphorylase by mutations in two different regulator genes involved in capsular polysaccharide synthesis in Escherichia coli K-12.
- Source :
-
Journal of bacteriology [J Bacteriol] 1970 Mar; Vol. 101 (3), pp. 965-72. - Publication Year :
- 1970
-
Abstract
- Mutations in a regulator gene (capR) that causes increased synthesis of capsular polysaccharide and derepressed synthesis of several enzymes involved in polysaccharide synthesis also derepress synthesis of guanosine diphosphate (GDP)-mannose pyrophosphorylase. In addition, a second mucoid mutation (capS, which maps separately from capR) also results in the derepression of GDP-mannose pyrophosphorylase. New conditions for assaying GDP-mannose hydrolyase and GDP-l-fucose synthetase permitted us to show that these enzymes are also derepressed in the capS mucoid strain. Although phosphomannose isomerase and uridine diphosphate-galactose-4-epimerase are derepressed in capR mucoid strains, they are not derepressed in capS mucoid strains. A nonmucoid mutant of a strain containing the capR9 (mucoid) allele was deficient in GDP-mannose pyrophosphorylase.
- Subjects :
- Escherichia coli metabolism
Genetics, Microbial
Glycoside Hydrolases metabolism
Guanine Nucleotides
Isomerases metabolism
Ligases metabolism
Nucleotidyltransferases metabolism
Uracil Nucleotides
Cell Wall metabolism
Escherichia coli enzymology
Genes, Regulator
Mutation
Polysaccharides, Bacterial biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9193
- Volume :
- 101
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 4908790
- Full Text :
- https://doi.org/10.1128/jb.101.3.965-972.1970