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Carbon dioxide fixation by extracts of Streptococcus faecalis var. liquefaciens.
- Source :
-
Journal of bacteriology [J Bacteriol] 1970 May; Vol. 102 (2), pp. 341-6. - Publication Year :
- 1970
-
Abstract
- Extracts of cells of Streptococcus faecalis var. liquefaciens strain 31 incorporated (14)CO(2) into aspartate. Dialyzed extracts produced radioactive oxalacetate in the absence of exogenously added glutamate and pyridoxal-5'-phosphate and produced radioactive aspartate in the presence of these components. Reduced nicotinamide adenine dinucleotide or reduced nicotinamide adenine dinucleotide phosphate could not be substituted for adenosine triphosphate (ATP); phosphoenolpyruvate even in the presence of nucleoside diphosphates could not replace pyruvate plus ATP; propionate plus coenzyme A (CoA) could not replace pyruvate in supporting CO(2) fixation by cell extracts. Fixation by dialyzed cell extracts required pyruvate, ATP, MgSO(4), and was stimulated by biotin, KCl, 2-mercaptoethanol, CoA, and acetyl CoA. Inhibition of fixation occurred when avidin, NaCl, oxalacetate, or aspartate was added to dialyzed extracts. On the basis of the products formed and the effects of substrates and cofactors on the fixation reaction, it was concluded that pyruvate carboxylase is responsible for CO(2) fixation in this microorganism.
- Subjects :
- Adenosine Triphosphate metabolism
Aspartic Acid biosynthesis
Bacteriological Techniques
Biotin pharmacology
Carbon Isotopes
Centrifugation
Chromatography, Paper
Coenzyme A pharmacology
Culture Media
Depression, Chemical
Dialysis
Enterococcus faecalis enzymology
Glutamates metabolism
Hydrogen-Ion Concentration
Ligases metabolism
Magnesium Sulfate metabolism
Mercaptoethanol pharmacology
Ovalbumin pharmacology
Oxaloacetates biosynthesis
Potassium Chloride pharmacology
Pyridoxal Phosphate metabolism
Pyruvates metabolism
Sodium Chloride pharmacology
Stimulation, Chemical
Carbon Dioxide metabolism
Enterococcus faecalis metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9193
- Volume :
- 102
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 4986758
- Full Text :
- https://doi.org/10.1128/jb.102.2.341-346.1970