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Isocitrate dehydrogenase and glutamate synthesis in Acetobacter suboxydans.
- Source :
-
Journal of bacteriology [J Bacteriol] 1969 Dec; Vol. 100 (3), pp. 1264-70. - Publication Year :
- 1969
-
Abstract
- Acetobacter suboxydans is an obligate aerobe for which an operative tricarboxylic acid cycle has not been demonstrated. Glutamate synthesis has been reported to occur by mechanisms other than those utilizing isocitrate dehydrogenase, a tricarboxylic acid cycle enzyme not previously detected in this organism. We have recovered alpha-ketoglutarate and glutamate from a system containing citrate, nicotinamide adenine dinucleotide (NAD), a divalent cation, pyridoxal phosphate, an amino donor, and dialyzed, cell-free extract. Aconitase activity was readily detected in these extracts, but isocitrate dehydrogenase activity, measured by NAD reduction, was masked by a cyanide-resistant, particulate, reduced NAD oxidase. Isocitrate dehydrogenase activity could be demonstrated after centrifuging the extracts at 150,000 x g for 3 hr and treating the supernatant fluid with 2-heptyl-4-hydroxyquinoline N-oxide. It is concluded that A. suboxydans can utilize the conventional tricarboxylic acid cycle enzymes to convert citrate to alpha-ketoglutarate which can then undergo a transamination to glutamate.
- Subjects :
- Carbon Isotopes
Cell-Free System
Chromatography, Paper
Citrates metabolism
Citrates pharmacology
Hydro-Lyases metabolism
Ketoglutaric Acids metabolism
Oxidoreductases metabolism
Acetobacter drug effects
Acetobacter enzymology
Acetobacter metabolism
Glutamates biosynthesis
Isocitrate Dehydrogenase metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9193
- Volume :
- 100
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 5361215
- Full Text :
- https://doi.org/10.1128/jb.100.3.1264-1270.1969