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Determination of L-alanosine in plasma and urine by reversed-phase high-performance liquid chromatography of the Dns derivative.
- Source :
-
Journal of chromatography [J Chromatogr] 1979 Feb 11; Vol. 170 (1), pp. 195-201. - Publication Year :
- 1979
-
Abstract
- L-Alanosine is an antitumour antibiotic that has recently been placed in clinical trial. We have developed a relatively rapid and specific assay for urinary and plasma alanosine, based on formation of the Dns derivative and separation of this from other Dns compounds by reversed-phase high-performance liquid chromatography. Dns-Alanosine is detected by its absorption at 254 nm, since alanosine is atypical in that it forms a Dns derivative with very low fluorescence. The lower limit of detection of alanosine in plasma is 0.1 microgram/ml. The assay has been used to measure the levels of alanosine in the plasma and urine of rabbits and of man.
- Subjects :
- Alanine analysis
Alanine blood
Alanine urine
Animals
Antibiotics, Antineoplastic blood
Antibiotics, Antineoplastic urine
Chromatography, High Pressure Liquid
Dansyl Compounds
Female
Humans
Male
Microchemistry
Nitrosamines analysis
Nitrosamines blood
Nitrosamines urine
Rabbits
Alanine analogs & derivatives
Antibiotics, Antineoplastic analysis
Subjects
Details
- Language :
- English
- Volume :
- 170
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of chromatography
- Publication Type :
- Academic Journal
- Accession number :
- 544618
- Full Text :
- https://doi.org/10.1016/s0021-9673(00)84251-x