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Somatic antigens of Streptococcus group E. II. Separation and a partial physicochemical characterization.
- Source :
-
Applied microbiology [Appl Microbiol] 1970 May; Vol. 19 (5), pp. 823-9. - Publication Year :
- 1970
-
Abstract
- Antigenic extracts of Streptococcus group E (SGE) were subjected to fractional ethanol precipitation, block (preparative) electrophoresis, and gel filtration for the purpose of separating the type antigen from the group antigen. Ethanol precipitation was ineffective in separating the substances. Block electrophoresis yielded serologically pure group antigen and a mixture of type and group antigen. Serologically pure type antigen was obtained by gel filtration. In some cases, pure group antigen was also recovered; in others, it was contaminated with type antigen. Gel filtration column effluents of antigenic extracts of SGE serotypes, I, II, III, IV, V and "untypable" isolates, collected from the region in which type antigen was eluted, were studied by paper chromatography and infrared spectrophotometry in an effort to develop a nonserological means of detecting type antigen. Hydrolysates of type antigens or suspect type antigens of serotypes I through V contained l-rhamnose, d-glucose, and several unidentified substances. d-Galactose also was present in hydrolysates of serotypes III and V. Untypable isolates and negative controls contained traces of d-glucose only. The data suggested that serotypes I through V contained a type antigen and that the untypable isolates were devoid of type antigen. Infrared absorbance spectra seemed to support the paper chromatography data.
- Subjects :
- Animals
Antigens analysis
Chemical Precipitation
Chromatography, Gel
Chromatography, Paper
Electrophoresis
Ethanol
Galactose analysis
Glucose analysis
Infrared Rays
Lymphadenitis immunology
Lymphadenitis veterinary
Precipitin Tests
Rhamnose analysis
Serotyping
Spectrophotometry
Streptococcal Infections immunology
Streptococcal Infections veterinary
Swine
Swine Diseases immunology
Antigens isolation & purification
Streptococcus immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0003-6919
- Volume :
- 19
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Applied microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 5463579
- Full Text :
- https://doi.org/10.1128/am.19.5.823-829.1970